PMID- 10568746
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190607
IS  - 1088-9051 (Print)
IS  - 1088-9051 (Linking)
VI  - 9
IP  - 11
DP  - 1999 Nov
TI  - A high-resolution physical map of human chromosome 21p using yeast artificial 
      chromosomes.
PG  - 1059-73
AB  - The short arm of human chromosome 21 (21p) contains many different types of 
      repetitive sequences and is highly homologous to the short arms of other 
      acrocentric chromosomes. Owing to its repetitive nature and the lack of 
      chromosome 21p-specific molecular markers, most physical maps of chromosome 21 
      exclude this region. We constructed a physical map of chromosome 21p using 
      sequence tagged site (STS) content mapping of yeast artificial chromosomes 
      (YACs). To this end, 39 STSs located on the short arm or near the centromere of 
      chromosome 21 were constructed, including four polymorphic simple tandem repeats 
      (STRs) and two expressed sequence tags (ESTs). Thirty YACs were selected from the 
      St. Louis YAC library, the chromosome 21-enriched ICRF YAC library, and the CEPH 
      YAC and megaYAC libraries. These were assembled in a YAC contig map ranging from 
      the centromere to the rDNA gene cluster at 21p12. The total size of the region 
      covered by YACs is estimated between 2.9 and 5 Mb. The integrity of the YAC 
      contig was confirmed by restriction enzyme fingerprinting and fluorescence in 
      situ hybridization (FISH). One gap with an estimated size of 400 kb remained near 
      the telomeric end of the contig. This YAC contig map of the short arm of human 
      chromosome 21 constitutes a basic framework for further structural and functional 
      studies of chromosome 21p.
FAU - Wang, S Y
AU  - Wang SY
AD  - Flanders Interuniversity Institute for Biotechnology (VIB), Born-Bunge Foundation 
      (BBS), Department of Biochemistry, University of Antwerp (UIA), B-2610 Antwerpen, 
      Belgium.
FAU - Cruts, M
AU  - Cruts M
FAU - Del-Favero, J
AU  - Del-Favero J
FAU - Zhang, Y
AU  - Zhang Y
FAU - Tissir, F
AU  - Tissir F
FAU - Potier, M C
AU  - Potier MC
FAU - Patterson, D
AU  - Patterson D
FAU - Nizetic, D
AU  - Nizetic D
FAU - Bosch, A
AU  - Bosch A
FAU - Chen, H
AU  - Chen H
FAU - Bennett, L
AU  - Bennett L
FAU - Estivill, X
AU  - Estivill X
FAU - Kessling, A
AU  - Kessling A
FAU - Antonarakis, S E
AU  - Antonarakis SE
FAU - van Broeckhoven, C
AU  - van Broeckhoven C
LA  - eng
SI  - GENBANK/AF187010
SI  - GENBANK/AF187011
GR  - HD17449/HD/NICHD NIH HHS/United States
GR  - HG00406/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genome Res
JT  - Genome research
JID - 9518021
SB  - IM
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 21/*genetics
MH  - Contig Mapping/methods
MH  - Expressed Sequence Tags
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Metaphase
MH  - Pedigree
MH  - *Physical Chromosome Mapping/methods
MH  - Sequence Tagged Sites
PMC - PMC310823
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
PMCR- 2000/05/01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
PHST- 2000/05/01 00:00 [pmc-release]
AID - 971R [pii]
AID - 10.1101/gr.9.11.1059 [doi]
PST - ppublish
SO  - Genome Res. 1999 Nov;9(11):1059-73. doi: 10.1101/gr.9.11.1059.