PMID- 10568761
OWN - NLM
STAT- MEDLINE
DCOM- 20000119
LR  - 20041117
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 60
IP  - 1
DP  - 2000 Jan
TI  - Human herpesvirus 6 infection in autologous bone marrow transplant recipients: a 
      prospective study.
PG  - 39-42
AB  - After primary infection in early life, human herpesvirus 6 (HHV-6) remains latent 
      in the body and may reactivate in subjects with poor immune status. A 180-day 
      longitudinal study of HHV-6 infection was carried out in 23 autologous bone 
      marrow transplant recipients to evaluate reactivation of HHV-6; two of these 
      patients underwent a double transplant. The patients were monitored prospectively 
      for HHV-6 DNA in peripheral blood mononuclear cells (PBMC) by hot start nested 
      PCR. Positive samples were typed by the enzymatic restriction protocol. Positive 
      plasma samples were also tested for HHV-6 DNA. Antibodies against HHV-6 were 
      measured by immunofluorescence. Five and two out of 23 patients had intermittent 
      and persistent positivity to HHV-6 DNA in PBMCs, respectively; four patients 
      carried variant B, and the other three patients both A and B. None of the 
      respective plasma samples were positive. Two patients were positive for HHV-6 
      antibodies. Since the significance of HHV-6 DNA in PBMCs is unclear, these 
      findings do not necessarily indicate active infection but may be due to mild 
      immunosuppression in autologous BMT recipients.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Moschettini, D
AU  - Moschettini D
AD  - Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita di 
      Siena, Siena, Italy. Moschettini@unisi.it
FAU - Galieni, P
AU  - Galieni P
FAU - Valensin, P E
AU  - Valensin PE
FAU - Laszlo, D
AU  - Laszlo D
FAU - Scalia, G
AU  - Scalia G
FAU - Tozzi, M
AU  - Tozzi M
FAU - Lauria, F
AU  - Lauria F
FAU - Donati, D
AU  - Donati D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Antibodies, Viral/blood
MH  - Bone Marrow Transplantation/*adverse effects
MH  - DNA, Viral/blood
MH  - Female
MH  - Herpesviridae Infections/immunology/*virology
MH  - Herpesvirus 6, Human/immunology/isolation & purification/*physiology
MH  - Humans
MH  - Leukocytes, Mononuclear/*virology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - Prospective Studies
MH  - Transplantation, Autologous
MH  - Virus Activation
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1002/(SICI)1096-9071(200001)60:1<39::AID-JMV7>3.0.CO;2-I [pii]
PST - ppublish
SO  - J Med Virol. 2000 Jan;60(1):39-42.