PMID- 10568840
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 30
IP  - 4
DP  - 1999 Dec 1
TI  - Structure and function of the methanogenic archaeal community in stable 
      cellulose-degrading enrichment cultures at two different temperatures (15 and 30 
      degrees C).
PG  - 313-326
AB  - Methanogenic cultures were enriched from an air-dried rice field soil and 
      incubated under anaerobic conditions at 30 degrees C with cellulose as substrate 
      (ET1). The culture was then transferred and further incubated at either 15 
      degrees C (E15) or 30 degrees C (E30), to establish stable cultures that 
      methanogenically degrade cellulose. After five transfers, the rates of CH(4) 
      production became reproducible. At 30 degrees C, CH(4) production rates were 
      (mean+/-S.D.) 15.2+/-0.7 nmol h(-1) ml(-1) culture for the next 16 transfers and 
      at 15 degrees C, they were 0.38+/-0.07 nmol h(-1) ml(-1) for the next six 
      transfers. When E30 was assayed at temperatures between 5-50 degrees C, CH(4) 
      production rates increased with the temperature, reached a maximum at 40 degrees 
      C and then decreased. The same temperature optimum was observed in E15, but with 
      a lower maximum CH(4) production rate. The apparent activation energies of CH(4) 
      production were similar (about 120 kJ mol(-1)4 mM at the beginning of the assay. 
      The structure of the archaeal community was analyzed by molecular techniques. 
      Total DNA was extracted from the microbial cultures before the transfer to 
      different temperatures (ET1) and afterwards (E15, E30). The archaeal small 
      subunit (SSU) ribosomal RNA-encoding genes (rDNA) of these DNA samples were 
      amplified by PCR with archaeal-specific primers and characterized by terminal 
      restriction fragment length polymorphism (T-RFLP). After obtaining a constant 
      T-RFLP pattern in the cultural transfers at 15 and 30 degrees C, the PCR 
      amplicons were used for the generation of clone libraries. Representative rDNA 
      clones (n=10 for each type of culture) were characterized by T-RFLP and sequence 
      analysis. In the primary culture (ET1), the archaeal community was dominated by 
      clones representing 'rice cluster I', a novel lineage of methanogenic 
      Euryarchaeota. However, further transfers resulted in the dominance of 
      Methanosarcinaceae and Methanosaetaceae at 30 and 15 degrees C, respectively. 
      This dominance was confirmed by fluorescence in situ hybridization (FISH) of 
      archaeal cells. Obviously, different archaeal communities were established at the 
      two different temperatures, but their activities nevertheless exhibited similar 
      temperature optima.
FAU - Chin, K
AU  - Chin K
AD  - Max-Planck-Institut fur terrestrische Mikrobiologie, Karl-von-Frisch-Str., 
      D-35043, Marburg, Germany
FAU - Lukow, T
AU  - Lukow T
FAU - Stubner, S
AU  - Stubner S
FAU - Conrad, R
AU  - Conrad R
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S0168649699000689 [pii]
AID - 10.1111/j.1574-6941.1999.tb00659.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 1999 Dec 1;30(4):313-326. doi: 
      10.1111/j.1574-6941.1999.tb00659.x.