PMID- 10570002
OWN - NLM
STAT- MEDLINE
DCOM- 19991221
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 61
IP  - 6
DP  - 1999 Dec
TI  - Retinoic acid receptors and retinoid X receptors in the rat testis during fetal 
      and postnatal development: immunolocalization and implication in the control of 
      the number of gonocytes.
PG  - 1548-57
AB  - Retinoids have pleiotropic effects on embryonic development and are essential for 
      spermatogenesis in the adult, where they act via nuclear retinoid receptors: 
      retinoic acid receptors (RARs) and retinoid X receptors (RXRs). We used 
      immunohistochemistry to examine the cellular localization of RARs and RXRs in the 
      rat testis from Day 13.5 postconception (13.5 dpc) until Day 8 postpartum (8 
      dpp), and these findings were compared with those for immature and adult testes. 
      RARalpha and RARbeta were detected in the interstitial tissue from 14.5 dpc, with 
      intense staining in the gonocytes from 20. 5 dpc to 8 dpp. The nuclei of all cell 
      types stained faintly for RARgamma from 8 dpp. Immunoreactivity for RXRalpha was 
      intense in the gonocytes from 13.5 dpc and in the Leydig cells from 16.5 dpc, and 
      persisted throughout the period studied. RXRbeta was always detected in the 
      Leydig cells and during a short neonatal period in the gonocytes. RXRgamma gave a 
      faint reaction in the nuclei of all cell types from 20.5 dpc. Unexpectedly, 
      immunostaining for all the receptors tested, except RARgamma and RXRgamma, was 
      detected in the cytoplasmic compartment of the cells of fetal and neonatal 
      testes, while it was found in the nuclei in immature and adult testes. In 
      cultures of dispersed testicular cells from 3 dpp pups, retinoic acid had a 
      dose-dependent deleterious effect on the survival of the gonocytes and, to a 
      lesser extent, of the somatic cells. These results suggest that retinoids act on 
      the testicular development, especially on germ cells, via RARs and/or RXRs.
FAU - Boulogne, B
AU  - Boulogne B
AD  - Universite Paris 7-INSERM-INRA U 418, Paris, France INSERM-INRA U 418, Hopital 
      Debrousse, Lyon, France.
FAU - Levacher, C
AU  - Levacher C
FAU - Durand, P
AU  - Durand P
FAU - Habert, R
AU  - Habert R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Retinoids)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Count
MH  - Cell Nucleus/chemistry
MH  - Cells, Cultured
MH  - Cytoplasm/chemistry
MH  - Immunohistochemistry
MH  - Leydig Cells/chemistry/ultrastructure
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Retinoic Acid/*analysis/*physiology
MH  - Retinoid X Receptors
MH  - Retinoids/pharmacology
MH  - Sertoli Cells/chemistry/ultrastructure
MH  - *Sperm Count
MH  - Spermatozoa/ultrastructure
MH  - Testis/embryology/*growth & development/ultrastructure
MH  - Transcription Factors/*analysis/*physiology
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1095/biolreprod61.6.1548 [doi]
PST - ppublish
SO  - Biol Reprod. 1999 Dec;61(6):1548-57. doi: 10.1095/biolreprod61.6.1548.