PMID- 10570206
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 24
DP  - 1999 Nov 23
TI  - G(o) protein-dependent survival of primary accessory olfactory neurons.
PG  - 14106-11
AB  - Extensive G protein-coupled receptor families in both the main and accessory 
      olfactory systems have been implicated in axonal targeting, sensory function, and 
      cell survival. Although sensory function seems to be mediated by G proteins, 
      axonal guidance and cell survival may be G protein-independent processes. In the 
      accessory olfactory system, the G(o)-containing neurons in the basal vomeronasal 
      organ (VNO) project to the posterior accessory olfactory bulb (AOB), whereas more 
      apically located VNO neurons contain G(i2) and project to the anterior AOB. 
      Herein, we investigate the organization of the accessory olfactory system in mice 
      with a targeted deletion in the G(o)alpha gene. The accessory olfactory system 
      seems normal at birth; however, postnatally, the number of 
      G(o)-receptor-containing VNO neurons decreases by half, and apoptotic neurons are 
      detected. The axons of VNO neurons remain restricted to the posterior AOB. The 
      posterior AOB is reduced in size but contains a synaptophysin-positive layer with 
      the normal number of glomeruli. The posterior AOB has reduced mitral cell c-Fos 
      immunoreactivity, consistent with decreased sensory activation of G(o) 
      protein-coupled VNO receptor neurons. Thus, in the accessory olfactory system, 
      receptor-coupled G proteins are required for cell survival.
FAU - Tanaka, M
AU  - Tanaka M
AD  - Department of Neurology, Yale University School of Medicine, New Haven, CT 06510, 
      USA.
FAU - Treloar, H
AU  - Treloar H
FAU - Kalb, R G
AU  - Kalb RG
FAU - Greer, C A
AU  - Greer CA
FAU - Strittmatter, S M
AU  - Strittmatter SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (GTP-Binding Protein alpha Subunits)
RN  - 0 (Ncam2 protein, mouse)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Synaptophysin)
RN  - 0 (olfactory G protein subunit alpha olf)
RN  - EC 3.6.5.1 (GNAI2 protein, human)
RN  - EC 3.6.5.1 (GTP-Binding Protein alpha Subunit, Gi2)
RN  - EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gi-Go)
RN  - EC 3.6.5.1 (Gnai2 protein, mouse)
RN  - EC 3.6.5.1 (Gnai2 protein, rat)
RN  - EC 3.6.5.1 (Heterotrimeric GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Adhesion Molecules, Neuronal/biosynthesis
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Female
MH  - GTP-Binding Protein alpha Subunit, Gi2
MH  - GTP-Binding Protein alpha Subunits
MH  - *GTP-Binding Protein alpha Subunits, Gi-Go
MH  - Heterotrimeric GTP-Binding Proteins/biosynthesis/genetics/*physiology
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - *Neural Cell Adhesion Molecules
MH  - Neurons/cytology/*metabolism
MH  - Proto-Oncogene Proteins/biosynthesis/genetics
MH  - Proto-Oncogene Proteins c-fos/biosynthesis
MH  - Rats
MH  - Synaptophysin/biosynthesis
MH  - Vomeronasal Organ/cytology/*metabolism
PMC - PMC24198
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
PMCR- 2000/05/23
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
PHST- 2000/05/23 00:00 [pmc-release]
AID - 2930 [pii]
AID - 10.1073/pnas.96.24.14106 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14106-11. doi: 
      10.1073/pnas.96.24.14106.