PMID- 10571521
OWN - NLM
STAT- MEDLINE
DCOM- 19991130
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 30
IP  - 11
DP  - 1999 Nov
TI  - Molecular cytogenetics of chromosome 11 in pituitary adenomas: a comparison of 
      fluorescence in situ hybridization and DNA ploidy study.
PG  - 1377-82
AB  - Chromosome 11 abnormalities were detected by fluorescence in situ hybridization 
      (FISH) technique and compared with DNA ploidy in 24 surgically removed pituitary 
      adenomas. The tumors were diagnosed and classified by histology, electron 
      microscopy, and pituitary hormone immunocytochemistry. They included 2 densely 
      granulated somatotroph (DG-SM) and 4 sparsely granulated somatotroph (SG-SM) 
      adenomas, 3 SG lactotroph (LT), 2 mixed somatotroph-lactotroph (SM-LT), 4 
      functioning corticotroph (CRT), 1 silent CRT subtype 1, 1 thyrotroph, 1 mixed 
      thyrotroph-somatotroph, 2 gonadotrophs, and 4 null cell adenomas. FISH analysis 
      with an alpha-satellite DNA probe specific for chromosome 11 showed numerical 
      abnormalities in 16 functioning (94%) and 5 nonfunctioning (71%) adenomas. Ten 
      functioning tumors showed aneuploid histograms, whereas the remaining and all 
      nonfunctioning adenomas were diploid. Aberrant chromosome 11 signals were noted 
      mostly in aneuploid adenomas involving 17% to 100% of their cell population. The 
      severity of chromosome 11 aberrations in adenomas containing extra copies often 
      correlated with a higher DNA index (DI). Monosomy 11 as dominant aberration was 
      noted in a mixed SM-LT and to a lesser degree in 3 CRT adenomas involving 21% to 
      97% of their cell population. Two of these CRT adenomas were associated with 
      normal DI, whereas the remaining third showed a high DI, indicating increased 
      copy number of chromosomes other than of chromosome 11. In conclusion, chromosome 
      11 abnormalities are common in all types of pituitary adenomas, occurring more 
      frequently in functioning tumors. Specific numerical abnormalities, such as 
      monosomy and trisomy, tend to be associated with certain adenoma types, whereas 
      tumors with extra chromosome 11 copies often exhibit aneuploid histograms.
FAU - Kontogeorgos, G
AU  - Kontogeorgos G
AD  - Department of Pathology, G. Gennimatas Athens General Hospital, Greece.
FAU - Kapranos, N
AU  - Kapranos N
FAU - Orphanidis, G
AU  - Orphanidis G
FAU - Rologis, D
AU  - Rologis D
FAU - Kokka, E
AU  - Kokka E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adenoma/*genetics
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - *Chromosomes, Human, Pair 11
MH  - DNA/analysis
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Pituitary Neoplasms/*genetics
MH  - *Ploidies
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - S0046-8177(99)90072-2 [pii]
AID - 10.1016/s0046-8177(99)90072-2 [doi]
PST - ppublish
SO  - Hum Pathol. 1999 Nov;30(11):1377-82. doi: 10.1016/s0046-8177(99)90072-2.