PMID- 10573219
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20190822
IS  - 0903-1936 (Print)
IS  - 0903-1936 (Linking)
VI  - 14
IP  - 4
DP  - 1999 Oct
TI  - Presence of substance P and neurokinin 1 receptors in human sputum macrophages 
      and U-937 cells.
PG  - 776-82
AB  - Tachykinins such as substance P (SP) may be involved in the pathogenesis of 
      inflammatory airway diseases such as asthma. This study investigated the presence 
      of SP and its receptor in the differentiated macrophage-like U-937 cell line and 
      in macrophages from sputum induced in healthy subjects (n=8). In situ 
      hybridization with digoxigenin-labelled sense and antisense complementary 
      ribonucleic acid (cRNA) probes was used to determine the expression of SP and its 
      receptor (neurokinin (NK)1 receptor). SP-immunoreactive material was detected 
      using a rabbit anti-SP antiserum and the alkaline phosphatase anti-alkaline 
      phosphatase technique. Beta-preprotachykinin (PPT)-I messenger ribonucleic acid 
      (mRNA) encoding SP, was detected using in situ hybridization in differentiated 
      U-937 cells as well as in CD45+ human leukocyte antigen (HLA) DR+ sputum 
      macrophages. The expression of the beta-PPT-I mRNA was increased in 
      lipopolysaccharide (LPS)-stimulated U-937 cells. SP-immunoreactive material was 
      found in differentiated U-937 cells and in CD68+ sputum macrophages. NK1 receptor 
      mRNA was detected in differentiated U-937 cells and sputum macrophages. 
      Incubation of U-937 cells with SP considerably increased the expression of NK1 
      receptor mRNA. This study demonstrates that human monocytes/macrophages express 
      substance P and that this expression is upregulated by lipopolysacharide. Human 
      monocytes/macrophages also express neurokinin1 receptor messenger ribonucleic 
      acid, suggesting an autocrine effect of substance P on these cells.
FAU - Germonpre, P R
AU  - Germonpre PR
AD  - Dept of Respiratory Diseases, University of Ghent, Belgium.
FAU - Bullock, G R
AU  - Bullock GR
FAU - Lambrecht, B N
AU  - Lambrecht BN
FAU - Van De Velde, V
AU  - Van De Velde V
FAU - Luyten, W H
AU  - Luyten WH
FAU - Joos, G F
AU  - Joos GF
FAU - Pauwels, R A
AU  - Pauwels RA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur Respir J
JT  - The European respiratory journal
JID - 8803460
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Neurokinin-1)
RN  - 0 (Tachykinins)
RN  - 0 (preprotachykinin)
RN  - 33507-63-0 (Substance P)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - In Situ Hybridization
MH  - Leukocyte Common Antigens/immunology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages, Alveolar/cytology/drug effects/*metabolism
MH  - Protein Precursors/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, Neurokinin-1/genetics/*metabolism
MH  - Sputum/*cytology
MH  - Substance P/pharmacology
MH  - Tachykinins/genetics/metabolism
MH  - U937 Cells/drug effects/*metabolism
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1034/j.1399-3003.1999.14d08.x [doi]
PST - ppublish
SO  - Eur Respir J. 1999 Oct;14(4):776-82. doi: 10.1034/j.1399-3003.1999.14d08.x.