PMID- 10574371
OWN - NLM
STAT- MEDLINE
DCOM- 19991221
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 10
IP  - 12
DP  - 1999 Aug 20
TI  - Additive effect of NOS inhibitor and neurotrophic factors on the survival of 
      injured Clarke's neurons.
PG  - 2569-73
AB  - The present study examined the effect of treatment with the NOS inhibitor 
      N(G)-nitro-L-arginine methyl ester (L-NAME) together with peripheral nerve (PN) 
      graft or brain-derived neurotrophic factor (BDNF) on the survival of CN neurons 
      at the L1 level of the spinal cord following hemisection at T11. In control 
      animals 41% of CN neurons survived 15 days after the hemisection, and 48% of 
      these expressed NOS. Treatment with either PN graft implantation or continuous 
      infusion of BDNF increased the survival rate of CN neurons to 70%; 70% of these 
      expressed NOS. Combined L-NAME and PN graft or L-NAME and BDNF improved the 
      rescue rate up to 94%, but only approximately 33% expressed NOS. Our results 
      suggest that the expression of NOS might adversely influence the neuroprotective 
      function of neurotrophic factors on injured CN neurons in the spinal cord.
FAU - Yick, L W
AU  - Yick LW
AD  - Department of Anatomy, Faculty of Medicine, The University of Hong Kong, Hong 
      Kong.
FAU - Wu, W
AU  - Wu W
FAU - So, K F
AU  - So KF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Neuroprotective Agents)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Survival/drug effects
MH  - Enzyme Inhibitors/*pharmacology
MH  - Female
MH  - NG-Nitroarginine Methyl Ester/*pharmacology
MH  - Neurons/*drug effects/pathology
MH  - Neuroprotective Agents/*pharmacology
MH  - Nitric Oxide Synthase/*antagonists & inhibitors
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1097/00001756-199908200-00024 [doi]
PST - ppublish
SO  - Neuroreport. 1999 Aug 20;10(12):2569-73. doi: 10.1097/00001756-199908200-00024.