PMID- 10575426
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 445
IP  - 2
DP  - 1999 Sep 30
TI  - Chromosome damage and aneuploidy detected by interphase multicolour FISH in 
      benzene-exposed shale oil workers.
PG  - 155-66
AB  - A multicolour tandem-labelling fluorescence in situ hybridization (FISH) 
      procedure was used to detect chromosome alterations in peripheral blood cells of 
      a group of Estonian petrochemistry workers. Twelve workers employed in benzene 
      production and five cokery workers, together with eight unexposed rural controls, 
      were enrolled in the study. The methodology employed, based on the in situ 
      hybridization of adjacent centromeric and pericentromeric regions, allowed the 
      simultaneous detection of both chromosome breakage, involving damage-prone 
      pericentromeric regions, and hyperploidy in interphase cells. Blood smears from 
      all subjects were hybridized with chromosome 1 specific probes, in order to 
      detect genotoxic damage in circulating lymphocytes and granulocytes. Moreover, 
      lymphocyte cultures were established, harvested 48 h following mitogen 
      stimulation and hybridized with the tandem chromosomes 1 and 9 probes. No 
      significant difference in the incidence of breakage was detected in the nucleated 
      cells of blood smears of exposed vs. control subjects. In contrast, modest but 
      significantly increased frequencies of breakage affecting both chromosomes 1 and 
      9 were observed in the cultured lymphocytes of the benzene-exposed workers 
      compared to the unexposed controls, suggesting an expression of premutagenic 
      lesions during the S-phase in vitro. Across the entire study group, the 
      frequencies of breakage affecting chromosomes 1 and 9 in the stimulated 
      lymphocytes were highly intercorrelated (p < 0.001). No significant difference 
      was found in the incidence of hyperploidy among the study groups, although a 
      tendency to higher values was observed in benzene-exposed workers. Although the 
      relatively small size of the study groups does not allow firm conclusions on the 
      role of occupational exposure, the observed patterns are suggestive of effects in 
      the benzene-exposed workers. This work also shows that tandem labelling FISH can 
      be usefully applied in human biomonitoring, allowing the simultaneous detection 
      of both hyperploidy and chromosome breakage at interphase in different cell 
      types.
FAU - Marcon, F
AU  - Marcon F
AD  - Laboratory of Comparative Toxicology and Ecotoxicology, Istituto Superiore di 
      Sanita, Rome, Italy.
FAU - Zijno, A
AU  - Zijno A
FAU - Crebelli, R
AU  - Crebelli R
FAU - Carere, A
AU  - Carere A
FAU - Veidebaum, T
AU  - Veidebaum T
FAU - Peltonen, K
AU  - Peltonen K
FAU - Parks, R
AU  - Parks R
FAU - Schuler, M
AU  - Schuler M
FAU - Eastmond, D
AU  - Eastmond D
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Coke)
RN  - 0 (Petroleum)
RN  - J64922108F (Benzene)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Benzene/*adverse effects
MH  - Cells, Cultured
MH  - *Chromosome Breakage
MH  - Chromosomes, Human, Pair 1/*drug effects
MH  - Chromosomes, Human, Pair 9/*drug effects
MH  - Coke/adverse effects/analysis
MH  - DNA Damage
MH  - Estonia
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/cytology/*drug effects
MH  - Middle Aged
MH  - Occupational Diseases/*etiology/genetics
MH  - Occupational Exposure/*adverse effects
MH  - Petroleum
EDAT- 1999/11/27 00:00
MHDA- 1999/11/27 00:01
CRDT- 1999/11/27 00:00
PHST- 1999/11/27 00:00 [pubmed]
PHST- 1999/11/27 00:01 [medline]
PHST- 1999/11/27 00:00 [entrez]
AID - S1383-5718(99)00122-9 [pii]
AID - 10.1016/s1383-5718(99)00122-9 [doi]
PST - ppublish
SO  - Mutat Res. 1999 Sep 30;445(2):155-66. doi: 10.1016/s1383-5718(99)00122-9.