PMID- 10578427
OWN - NLM
STAT- MEDLINE
DCOM- 20000103
LR  - 20181130
IS  - 0028-2162 (Print)
IS  - 0028-2162 (Linking)
VI  - 143
IP  - 45
DP  - 1999 Nov 6
TI  - [Fluorescence in situ hybridization in the study of chromosomal abnormalities].
PG  - 2265-8
AB  - Classical cytogenetics has a low resolving power and allows analysis of dividing 
      cells only. In fluorescence in situ hybridization (FISH), a DNA fragment is 
      stained with a fluorescent marker, after which this fragment is brought into 
      contact with a patient's DNA. The stained fragment can bind to a corresponding 
      fragment, revealing its presence or absence. Using FISH, every desired DNA 
      sequence (from a whole chromosome to one gene) can be stained. In this way it is 
      also possible to diagnose microdeletion syndromes, such as the Williams syndrome, 
      the DiGeorge syndrome and submicroscopic chromosome anomalies that play a part in 
      mental handicaps. FISH also allows analysis of non-dividing cells. In this way it 
      is possible for instance rapidly to examine uncultured amniotic fluid cells for 
      the commoner trisomies or to find foetal erythrocytes in a pregnant woman's 
      blood. It is also possible to demonstrate tumour-specific breaking points. By 
      application of FISH to microarrays it is possible to study a large number of 
      genes simultaneously for the presence of a particular number of DNA sequences 
      linked to a clinical abnormality.
FAU - Hoovers, J M
AU  - Hoovers JM
AD  - Academisch Medisch Centrum, afd. Klinische Genetica, Amsterdam.
FAU - Mellink, C H
AU  - Mellink CH
FAU - Leschot, N J
AU  - Leschot NJ
LA  - dut
PT  - Journal Article
PT  - Review
TT  - Fluorescentie-in-situhybridisatie bij het onderzoek naar chromosomale 
      afwijkingen.
PL  - Netherlands
TA  - Ned Tijdschr Geneeskd
JT  - Nederlands tijdschrift voor geneeskunde
JID - 0400770
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosome Aberrations/*diagnosis/*genetics
MH  - Chromosome Deletion
MH  - Chromosome Disorders
MH  - Cytogenetic Analysis
MH  - *DNA Mutational Analysis
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*statistics & numerical data
MH  - Microsatellite Repeats
MH  - Syndrome
MH  - Translocation, Genetic
MH  - Trisomy/diagnosis/genetics
RF  - 13
EDAT- 1999/12/01 00:00
MHDA- 1999/12/01 00:01
CRDT- 1999/12/01 00:00
PHST- 1999/12/01 00:00 [pubmed]
PHST- 1999/12/01 00:01 [medline]
PHST- 1999/12/01 00:00 [entrez]
PST - ppublish
SO  - Ned Tijdschr Geneeskd. 1999 Nov 6;143(45):2265-8.