PMID- 10580796
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20190826
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 101
IP  - 2
DP  - 1999 Nov 15
TI  - Mast cell production of TNF-alpha induced by substance P evidence for a 
      modulatory role of substance P-antagonists.
PG  - 128-36
AB  - Unregulated increasing of Tumor necrosis factor-alpha (TNF-alpha) could be 
      pathogenic in inflammatory diseases. The aim of this study was to investigate the 
      anti-inflammatory role of the Substance P-antagonists (SPAs) through the 
      inhibition of histamine release (HR) and TNF-alpha production from mast cell. Rat 
      peritoneal mast cells (PMC) stimulated with Substance P (SP), in the presence of 
      SPAs or not, were analyzed for HR and TNF-alpha protein production. Competitive 
      Polymerase Chain Reaction, with an internal standard competing with target cDNA 
      for the same primers, was used to determine the TNF-alpha mRNA expression. We 
      show that the increase of either HR and TNF-alpha levels in peritoneal (PMC) 
      after induction with SP was inhibited by pre-incubation with SPA or with the 
      Peptide 101 (P101), while the [D-Pro2, D-Phe7, D-Trp9]-SP (dSP) had no effect. 
      Neuraminidase treatment suggests that dSP, as well as SP, interacts with sialic 
      acid residues on the cell surface. Moreover, SPA and P101 also inhibit the 
      release of histamine and TNF-alpha induced by dSP suggesting that a 
      receptor-independent mechanism is involved. These data could be useful to better 
      understand the mechanisms involved in the mast cell activation and TNF-alpha 
      production in the inflammatory diseases where SP is involved.
FAU - Cocchiara, R
AU  - Cocchiara R
AD  - Istituto di Biologia dello Sviluppo, CNR, Palermo, Italy.
FAU - Lampiasi, N
AU  - Lampiasi N
FAU - Albeggiani, G
AU  - Albeggiani G
FAU - Bongiovanni, A
AU  - Bongiovanni A
FAU - Azzolina, A
AU  - Azzolina A
FAU - Geraci, D
AU  - Geraci D
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 33507-63-0 (Substance P)
RN  - EC 3.2.1.18 (Neuraminidase)
SB  - IM
MH  - Animals
MH  - Female
MH  - Histamine Release/drug effects
MH  - Mast Cells/*drug effects/metabolism
MH  - Neuraminidase/pharmacology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Substance P/*antagonists & inhibitors/*pharmacology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/genetics
EDAT- 1999/12/02 09:00
MHDA- 2000/06/01 09:00
CRDT- 1999/12/02 09:00
PHST- 1999/12/02 09:00 [pubmed]
PHST- 2000/06/01 09:00 [medline]
PHST- 1999/12/02 09:00 [entrez]
AID - S0165572899001381 [pii]
AID - 10.1016/s0165-5728(99)00138-1 [doi]
PST - ppublish
SO  - J Neuroimmunol. 1999 Nov 15;101(2):128-36. doi: 10.1016/s0165-5728(99)00138-1.