PMID- 10581410
OWN - NLM
STAT- MEDLINE
DCOM- 20000214
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 73
IP  - 1-2
DP  - 1999 Nov 10
TI  - The mouse GABA(A) receptor alpha3 subunit gene and promoter.
PG  - 172-80
AB  - Gamma-aminobutyric acid (GABA) type A receptors are multisubunit ligand-gated ion 
      channels which mediate inhibition in the brain. The GABA(A) receptor alpha3 
      subunit gene exhibits extensive variation in its developmental and regional 
      expression, but the detailed mechanisms governing the expression patterns of this 
      gene remain unknown. We have cloned and begun to characterize the murine alpha3 
      subunit gene Gabra3. All but one of the 10 exons and the intron-exon boundaries 
      have been sequenced; the first intron is in the 5' untranslated region (5'UTR) of 
      the alpha3 mRNA. Rapid amplification of the cDNA 5'-end (5'-RACE) and RNase 
      protection indicated many transcription start sites, with the major site (=+1) 
      corresponding to a 5'UTR of 178 bases. Most sites were in or just downstream of a 
      region of 55 (mouse) and 25 (human) GA repeats in the proximal promoter, as 
      revealed by genome walking of Gabra3 and the human gene GABRA3. No canonical TATA 
      or CAAT boxes or initiator (Inr) sites were found in either promoter, but both 
      contained conserved consensus sites for several transcription factors. 
      Progressive deletion of the mouse promoter produced positive or negative effects 
      on expression of reporter (luciferase) constructs, with the highest observed 
      activity in several types of transiently transfected cells for a construct 
      containing bases -320 to +35. The GA repeats and a much shorter nearby series of 
      four GC repeats, the first three of which are part of a consensus E2F site, 
      appear to contribute significantly to mouse promoter activity. Upstream GA 
      repeats enhanced activity of the SV40 promoter, and the GA repeat sequence bound 
      nuclear proteins from several tissues.
FAU - Mu, W
AU  - Mu W
AD  - Department of Pharmacology and Experimental Therapeutics, University of Maryland 
      School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201-1559, USA.
FAU - Burt, D R
AU  - Burt DR
LA  - eng
GR  - HD16596/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Nuclear Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, GABA-A)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Base Sequence
MH  - Brain/metabolism
MH  - Cloning, Molecular
MH  - DNA/genetics/metabolism
MH  - Exons
MH  - Gene Expression Regulation
MH  - Genes/genetics
MH  - Introns
MH  - Liver/metabolism
MH  - Luciferases/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Promoter Regions, Genetic/*genetics
MH  - Protein Binding
MH  - Protein Isoforms/genetics
MH  - Receptors, GABA-A/*genetics
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Repetitive Sequences, Nucleic Acid/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Simian virus 40/genetics
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1999/12/03 09:00
MHDA- 2000/02/19 09:00
CRDT- 1999/12/03 09:00
PHST- 1999/12/03 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 1999/12/03 09:00 [entrez]
AID - S0169328X99002582 [pii]
AID - 10.1016/s0169-328x(99)00258-2 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Nov 10;73(1-2):172-80. doi: 
      10.1016/s0169-328x(99)00258-2.