PMID- 10582319 OWN - NLM STAT- MEDLINE DCOM- 20000215 LR - 20190915 IS - 1096-4959 (Print) IS - 1096-4959 (Linking) VI - 124 IP - 1 DP - 1999 Sep TI - Identification of differentially expressed genes in Con A-activated carp (Cyprinus carpio L.) leucocytes. PG - 41-50 AB - A cDNA library was constructed from the message RNA (mRNA) obtained from Con A-induced head kidney (HK) leucocytes of carp (Cyprinus carpio L.). Differential screening of the cDNA was carried out by hybridization against the total cDNA probes from normal, Con A-uninduced HK leucocytes or Con A-induced HK leucocytes of carp. The differential expression patterns of certain cDNA clones were confirmed by Southern-blot and Northern-blot analysis. Single-pass of the sequencing analysis and homology search in Genbank (EMBL) revealed those differentially expressed cDNA clones encode for cytochrome c oxidase sub-unit II and III (COII and COIII), elongation factor-1 beta (EF-1 beta), bleomycin hydrolase (BH), heat shock cognate protein 70 (HSC70) and 16S ribosomal RNA (16S rRNA). FAU - Yin, Z AU - Yin Z AD - Department of Biological Sciences, National University of Singapore, Singapore. FAU - He, J Y AU - He JY FAU - Gong, Z AU - Gong Z FAU - Lam, T J AU - Lam TJ FAU - Sin, Y M AU - Sin YM LA - eng SI - GENBANK/AF001098 SI - GENBANK/AF003110 SI - GENBANK/AF003111 SI - GENBANK/AF003112 PT - Comparative Study PT - Journal Article PL - England TA - Comp Biochem Physiol B Biochem Mol Biol JT - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JID - 9516061 RN - 0 (Carrier Proteins) RN - 0 (DNA, Recombinant) RN - 0 (HSC70 Heat-Shock Proteins) RN - 0 (HSP70 Heat-Shock Proteins) RN - 0 (HSPA8 protein, human) RN - 0 (Peptide Elongation Factor 1) RN - 0 (RNA, Ribosomal, 16S) RN - 11028-71-0 (Concanavalin A) RN - EC 1.9.3.1 (Electron Transport Complex IV) RN - EC 3.4.22.- (Cysteine Endopeptidases) SB - IM MH - Amino Acid Sequence MH - Animals MH - Blotting, Northern MH - Carps/*genetics/immunology MH - Carrier Proteins/genetics/immunology MH - Cloning, Molecular MH - Concanavalin A/*metabolism MH - Cysteine Endopeptidases/genetics/immunology MH - DNA, Recombinant MH - Electron Transport Complex IV/genetics/immunology MH - Gene Expression Profiling MH - Gene Expression Regulation MH - Gene Library MH - Genes/*genetics MH - HSC70 Heat-Shock Proteins MH - *HSP70 Heat-Shock Proteins MH - Humans MH - Leukocytes/*metabolism MH - *Lymphocyte Activation/genetics MH - Molecular Sequence Data MH - Peptide Elongation Factor 1/genetics/immunology MH - RNA, Ribosomal, 16S/genetics/immunology MH - Sequence Analysis, DNA MH - Sequence Homology, Amino Acid EDAT- 1999/12/03 09:00 MHDA- 2000/02/19 09:00 CRDT- 1999/12/03 09:00 PHST- 1999/12/03 09:00 [pubmed] PHST- 2000/02/19 09:00 [medline] PHST- 1999/12/03 09:00 [entrez] AID - 10.1016/s0305-0491(99)00095-4 [doi] PST - ppublish SO - Comp Biochem Physiol B Biochem Mol Biol. 1999 Sep;124(1):41-50. doi: 10.1016/s0305-0491(99)00095-4.