PMID- 10583222
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 107
IP  - 2
DP  - 1999 Nov
TI  - Characterization of 12p molecular events outside ETV6 in complex karyotypes of 
      acute myeloid malignancies.
PG  - 340-6
AB  - Acute myeloid disorders with rearrangements of 12p outside the ETV6 gene were 
      characterized by fluorescence in situ hybridization (FISH) with a panel of DNA 
      probes. Seven patients with de novo acute myeloid leukaemia (AML), one with 
      secondary acute myeloid leukaemia (sAML), and one in the blast phase of chronic 
      myeloid leukaemia (CML-BP) were enrolled in the study. All AML cases showed 
      multiple karyotypic changes. Chromosome 5 and/or 7 deletions were the most 
      frequent accompanying changes. FISH revealed amplification, cryptic 
      translocation, and fragmentation of chromosome 12, not discernible at karyotypic 
      level. Different karyotypic rearrangements of 12p showed a common molecular 
      event. Among the seven cases in which breakpoints could be determined, six were 
      telomeric and one centromeric to ETV6. In three AML cases a new recurrent 
      breakpoint in the telomeric region was identified distally to locus D12S158 and 
      to pac 922B22 which is the most telomeric probe available for 12p. Accompanying 
      cryptic deletions were also detected in five patients and the commonly deleted 
      region, of around 700 kb, included the ETV6 gene and the D12S391 locus.
FAU - La Starza, R
AU  - La Starza R
AD  - Haematology and Bone Marrow Transplantation Unit, University of Perugia, Perugia, 
      Italy.
FAU - Stella, M
AU  - Stella M
FAU - Testoni, N
AU  - Testoni N
FAU - Di Bona, E
AU  - Di Bona E
FAU - Ciolli, S
AU  - Ciolli S
FAU - Marynen, P
AU  - Marynen P
FAU - Martelli, M F
AU  - Martelli MF
FAU - Mandelli, F
AU  - Mandelli F
FAU - Mecucci, C
AU  - Mecucci C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosome Breakage/genetics
MH  - Chromosomes, Human, Pair 12
MH  - Female
MH  - Gene Rearrangement/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid/*genetics
MH  - Male
MH  - Middle Aged
MH  - Translocation, Genetic/genetics
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
AID - bjh1724 [pii]
AID - 10.1046/j.1365-2141.1999.01724.x [doi]
PST - ppublish
SO  - Br J Haematol. 1999 Nov;107(2):340-6. doi: 10.1046/j.1365-2141.1999.01724.x.