PMID- 10583479
OWN - NLM
STAT- MEDLINE
DCOM- 19991228
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 11
IP  - 11
DP  - 1999 Nov
TI  - Nerve growth factor (NGF) induces motoneuron apoptosis in rat embryonic spinal 
      cord in vitro.
PG  - 3904-12
AB  - Recent studies have demonstrated that nerve growth factor (NGF) induces apoptosis 
      of several cell types in the central nervous system through its low-affinity p75 
      neurotrophin receptor (p75NTR). To test the effect of NGF on embryonic motoneuron 
      survival, we developed an organotypic culture system which allowed the in vitro 
      development of intact embryonic rat spinal cords. In our system, neural tubes 
      were taken and cultured at E13, just before the onset of physiological motoneuron 
      death. After 2 days in vitro (DIV), motoneurons underwent apoptosis over a 
      time-course similar to that in vivo. In this system, the addition of NGF (200 
      ng/mL) for 2 days enhanced the number of apoptotic motoneurons by 37%. This 
      pro-apoptotic effect was completely reversed by the blocking anti-p75NTR (REX) 
      antibody which inhibits NGF binding to p75NTR. Other neurotrophins, e.g. 
      brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT3) and neurotrophin 
      4/5 (NT4/5) did not have any effect, while glial cell-derived neurotrophic factor 
      (GDNF) promoted motoneuron survival. Anti-BDNF blocking antibodies enhanced 
      motoneuron death indicating that endogenous BDNF promotes motoneuron survival in 
      explants. Our results demonstrate, for the first time, that NGF can induce 
      embryonic motoneuron apoptosis through its receptor p75NTR.
FAU - Sedel, F
AU  - Sedel F
AD  - Laboratoire de Biologie Cellulaire de la Synapse Normale et Pathologique (INSERM 
      U497), Ecole Normale Superieure, 46 rue d'Ulm, 75005 Paris, France.
FAU - Bechade, C
AU  - Bechade C
FAU - Triller, A
AU  - Triller A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Antibodies)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Receptors, Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Apoptosis/*drug effects
MH  - Cell Division
MH  - Cell Survival/drug effects
MH  - Embryo, Mammalian
MH  - Gestational Age
MH  - Motor Neurons/cytology/*drug effects/physiology
MH  - Nerve Growth Factors/genetics/*pharmacology
MH  - Neurotrophin 3/genetics
MH  - Organ Culture Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Nerve Growth Factor/antagonists & inhibitors/genetics/physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spinal Cord/cytology/*embryology
MH  - Transcription, Genetic
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
AID - ejn814 [pii]
AID - 10.1046/j.1460-9568.1999.00814.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 1999 Nov;11(11):3904-12. doi: 10.1046/j.1460-9568.1999.00814.x.