PMID- 10583591
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 98
IP  - 3
DP  - 1999 Nov
TI  - Low dose of orally administered antigen down-regulates the T helper type 
      2-response in a murine model of dust mite hypersensitivity.
PG  - 338-44
AB  - One of the main goals of immunotherapy of allergic diseases is the 
      down-regulation of the type I hypersensitivity reaction. We investigated in this 
      study the effect of oral administration of varying doses (0.25, 1.0, 4.0 and 10 
      mg) of dust mite extract (Dermatophagoides pteronyssinus, Dp) in sensitized A/Sn 
      mice. A marked decrease of the allergen-specific immunoglobulin E (IgE) response 
      was observed with all antigen doses. The mice orally tolerized with low Dp dose 
      (0.25 mg) had a significant decrease in the total serum IgE and in the 
      immunoglobulin G1 (IgG1), IgG2a and IgG2b antibody levels. The higher Dp dose 
      (10.0 mg), however, enhanced the IgG1 antibody response, suggesting the 
      stimulation of a pre-existing immune response of the sensitized animals. Animals 
      fed with the low Dp dose had a significant decrease in the frequency of 
      interleukin-4 (IL-4) secreting cells. These animals also showed a significant 
      decrease in the frequency of Dp-specific IgE- and IgG1-positive plasma cells. Our 
      data suggest that feeding dust mite extract to Dp-sensitized mice down-regulates 
      the development of type I hypersensitivity, by inhibition of the T helper 2 
      response.
FAU - Sato, M N
AU  - Sato MN
AD  - Laboratorio de Alergia e Imunologia Clinica e Experimental-LIM 56, Faculdade de 
      Medicina da Universidade de Sao Paulo, Sao Paulo, Brazil.
FAU - Carvalho, A F
AU  - Carvalho AF
FAU - Silva, A O
AU  - Silva AO
FAU - MacIel, M Jr
AU  - MacIel M Jr
FAU - Fusaro, A E
AU  - Fusaro AE
FAU - Duarte, A J
AU  - Duarte AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antigens/*administration & dosage
MH  - Antigens, Dermatophagoides
MH  - *Desensitization, Immunologic
MH  - Drug Administration Schedule
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Glycoproteins/*administration & dosage
MH  - Hypersensitivity, Immediate/immunology/*therapy
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Interleukin-4/blood
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Plasma Cells/immunology
MH  - Th2 Cells/*immunology
PMC - PMC2326944
EDAT- 1999/12/03 09:00
MHDA- 2001/03/28 10:01
PMCR- 2000/11/01
CRDT- 1999/12/03 09:00
PHST- 1999/12/03 09:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/12/03 09:00 [entrez]
PHST- 2000/11/01 00:00 [pmc-release]
AID - imm869 [pii]
AID - 10.1046/j.1365-2567.1999.00869.x [doi]
PST - ppublish
SO  - Immunology. 1999 Nov;98(3):338-44. doi: 10.1046/j.1365-2567.1999.00869.x.