PMID- 10583595
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20191210
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 98
IP  - 3
DP  - 1999 Nov
TI  - Nitric oxide and macrophage antiviral extrinsic activity.
PG  - 363-70
AB  - In this study we evaluated the relationship between nitric oxide (NO) and 
      macrophage antiviral extrinsic activity. Macrophages activated by intraperitoneal 
      injection of herpes simplex virus-2 (HSV-2), showed both extrinsic antiviral 
      activity and high nitrite production in contrast to non-activated, resident 
      macrophages. The extrinsic antiviral activity was observed in cultures of Vero 
      cells infected with HSV-1 and HSV-2. The NO inhibitor N-monomethyl-l-arginine 
      acetate (l-NMA) impaired the antiviral activity of HSV-elicited macrophages. The 
      effect was dose dependent and correlated with a reduction of nitrite in the 
      culture media. The effect of l-NMA was reversed by the addition of l-arginine. 
      These data indicate that NO could be responsible for the described activity. 
      Furthermore, l-NMA treatment resulted in the aggravation of HSV-1-induced 
      keratitis in the mouse model, supporting a defensive role of NO in the 
      pathogenesis of HSV-1 corneal infection.
FAU - Benencia, F
AU  - Benencia F
AD  - Department of Biological Chemistry, Faculty of Sciences, University of Buenos 
      Aires, Ciudad Universitaria, Argentina.
FAU - Courreges, M C
AU  - Courreges MC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Nitrites)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Herpesvirus 1, Human
MH  - Herpesvirus 2, Human/*immunology
MH  - Keratitis, Herpetic/*immunology/metabolism
MH  - *Macrophage Activation
MH  - Macrophages, Peritoneal/drug effects/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nitric Oxide/antagonists & inhibitors/*metabolism
MH  - Nitrites/metabolism
MH  - Vero Cells
MH  - Virus Replication/*immunology
MH  - omega-N-Methylarginine/pharmacology
PMC - PMC2326934
EDAT- 1999/12/03 00:00
MHDA- 1999/12/03 00:01
PMCR- 2000/11/01
CRDT- 1999/12/03 00:00
PHST- 1999/12/03 00:00 [pubmed]
PHST- 1999/12/03 00:01 [medline]
PHST- 1999/12/03 00:00 [entrez]
PHST- 2000/11/01 00:00 [pmc-release]
AID - imm864 [pii]
AID - 10.1046/j.1365-2567.1999.00864.x [doi]
PST - ppublish
SO  - Immunology. 1999 Nov;98(3):363-70. doi: 10.1046/j.1365-2567.1999.00864.x.