PMID- 10585395
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 50
DP  - 1999 Dec 10
TI  - Cullin 4A associates with the UV-damaged DNA-binding protein DDB.
PG  - 35309-12
AB  - The damaged DNA-binding protein (DDB) is believed to be involved in DNA repair, 
      and it has been linked to the repair deficiency disease xeroderma pigmentosum. 
      DDB also exhibits transcriptional activities. DDB binds to the activation domain 
      of E2F1 and stimulates E2F1-activated transcription. Here we provide evidence 
      that DDB or DDB-associated proteins are targets of cullin 4A (CUL-4A). CUL-4A is 
      a member of the cullin family of proteins, which are believed to be 
      ubiquitin-protein isopeptide ligases (type E3). The CUL-4A gene has been shown to 
      be amplified and up-regulated in breast carcinomas. In this study, we identify 
      CUL-4A as one of the DDB-associated proteins. CUL-4A co-immunoprecipitates with 
      DDB, but not with a naturally occurring mutant of DDB. Moreover, CUL-4A in HeLa 
      nuclear extracts co-purifies with DDB, suggesting they are parts of the same 
      complex. The observation provides insights how CUL-4A, through an interaction 
      with DDB, might be playing a role in the development of breast carcinomas.
FAU - Shiyanov, P
AU  - Shiyanov P
AD  - Department of Biochemistry, University of Illinois, Chicago, Illinois 60612, USA.
FAU - Nag, A
AU  - Nag A
FAU - Raychaudhuri, P
AU  - Raychaudhuri P
LA  - eng
GR  - CA76276/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CUL4A protein, human)
RN  - 0 (Cullin Proteins)
RN  - 0 (DDB1 protein, human)
RN  - 0 (DDB2 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Nucleus/metabolism
MH  - Chromatography, Affinity
MH  - *Cullin Proteins
MH  - *DNA Repair
MH  - DNA-Binding Proteins/chemistry/isolation & purification/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Neoplasm Proteins/chemistry/isolation & purification/*metabolism
MH  - Osteosarcoma
MH  - Recombinant Proteins/chemistry/isolation & purification/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - *Ultraviolet Rays
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - S0021-9258(19)53246-X [pii]
AID - 10.1074/jbc.274.50.35309 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 10;274(50):35309-12. doi: 10.1074/jbc.274.50.35309.