PMID- 10586940
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20151119
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 40
IP  - 13
DP  - 1999 Dec
TI  - Macrophages and dendritic cells in IRBP-induced experimental autoimmune 
      uveoretinitis in B10RIII mice.
PG  - 3177-85
AB  - PURPOSE: To investigate the characteristics of the mononuclear cell infiltrate in 
      murine experimental autoimmune uveoretinitis (EAU). METHODS: EAU was induced by 
      immunization with bovine interphotoreceptor retinal binding protein (IRBP) in 
      Freund's complete adjuvant (subcutaneous injection) and pertussis toxin 
      (intraperitoneal injection) in B10RIII mouse. Then animals were killed on days 7, 
      9, 12, 15, 20, 26, and 39 after immunization. Eyes were processed for hematoxylin 
      and eosin staining to characterize the disease and to assess the severity and 
      extent of the EAU. Single and dual immunohistochemical staining in various 
      combinations with monoclonal antibodies against CD45, CD4, CD8, major 
      histocompatibility complex (MHC) class II, CD11c, NLDC-145, and a variety of 
      macrophage markers was performed. RESULTS: The authors' results showed that 
      vitritis, vasculitis and perivasculitis, retinal detachment, and granuloma 
      formation in retina and choroid were the predominant features of IRBP-induced 
      B10RIII mice EAU. Immunohistologic results showed that CD4+ T cells and 
      macrophages were the main infiltrating cells in retina and choroid throughout the 
      entire course of the disease. MHC class II negative macrophages expressing 
      antigens reacting with MOMA-2, F4/80, sialoadhesin, and CD11b were prominent 
      during the peak phase of tissue damage in the retina and choroid. Dendritic cells 
      (DCs) characterized by dual positivity for MHC class II and CD11c and negative 
      for sialoadhesin appeared at time of disease onset and continued to be recruited 
      during the inflammatory process. DCs at the site of inflammation were NLDC-145 
      weak and CD8 negative, indicating that they were of the myeloid rather than the 
      lymphoid lineage. CONCLUSIONS: The results suggest that EAU in B10RIII mice is 
      initiated by local-infiltrating, dendritic antigen-presenting cells, whereas 
      tissue damage is associated with sialoadhesin-positive, phagocytic 
      nonantigen-presenting macrophages during the effector stage.
FAU - Jiang, H R
AU  - Jiang HR
AD  - Department of Ophthalmology, University of Aberdeen Medical School Foresterhill, 
      Scotland, United Kingdom.
FAU - Lumsden, L
AU  - Lumsden L
FAU - Forrester, J V
AU  - Forrester JV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers)
RN  - 0 (Eye Proteins)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Retinol-Binding Proteins)
RN  - 0 (interstitial retinol-binding protein)
SB  - IM
MH  - Animals
MH  - Antigens, CD/analysis
MH  - Autoimmune Diseases/chemically induced/*pathology
MH  - Biomarkers/analysis
MH  - CD4-Positive T-Lymphocytes/pathology
MH  - Cell Movement
MH  - Dendritic Cells/chemistry/*pathology
MH  - Disease Models, Animal
MH  - Eye Proteins
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Histocompatibility Antigens Class II/analysis
MH  - Immunoenzyme Techniques
MH  - Immunophenotyping
MH  - Macrophages/chemistry/*pathology
MH  - Male
MH  - Mice
MH  - Retinitis/chemically induced/*pathology
MH  - Retinol-Binding Proteins
MH  - Uveitis, Posterior/chemically induced/*pathology
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1999 Dec;40(13):3177-85.