PMID- 10589154
OWN - NLM
STAT- MEDLINE
DCOM- 20000107
LR  - 20141120
IS  - 0341-6593 (Print)
IS  - 0341-6593 (Linking)
VI  - 106
IP  - 10
DP  - 1999 Oct
TI  - [Effect of unfractionated and low-molecular-weight heparin on platelet 
      aggregation and in vitro bleeding time in dogs].
PG  - 439-44
AB  - The aim of the present study was to examine the influence of unfractionated 
      heparin (UFH) and low molecular weight heparin (LMWH) on the function of canine 
      thrombocytes. This was performed by adding different concentrations of UFH or 
      LMWH to platelet rich plasma (PRP) or blood of healthy dogs: 0.2, 0.5, 1, 2, 5, 
      10, 20, and 50 I.U./ml UFH or Anti-FXaU/ml LMWH, respectively (aggregation 
      induced by thrombin additionally: 0.025, 0.05, and 0.1 I.U./ml UFH or 
      Anti-FXaU/ml LMWH.) Platelet aggregation induced by ADP, collagen or thrombin 
      with the BORN method (n = 11) as well as the in vitro bleeding time using the 
      analyzer PFA-100 (n = 5) were examined. Additionally, a specific test assay for 
      ADP induced platelet aggregation was performed which enabled an individual 
      adjustment of the aggregation maximum at 30-40% in the control measurements (n = 
      6). The most prominent effect was noted in the platelet aggregation induced by 
      thrombin. The aggregation maximum of the platelet aggregation induced by 1 
      I.U./ml thrombin (final concentration) was significantly lower in all of the 
      testet UFH concentrations > or = 0.025 I.U./ml UFH in comparison to control 
      measurements. If the aggregation was induced by 10 I.U./ml thrombin a significant 
      reduction of the aggregation maximum was restricted to UFH concentrations > or = 
      0.5 I.U./ml. The addition of LMWH to canine PRP resulted in a distinct decrease 
      (p < 0.01) of the maximum aggregation induced by 1 I.U./ml thrombin in 
      concentrations > or = 0.2 Anti-FXaU/ml LMWH. A slight decrease of the maximum 
      aggregation induced by collagen was only found for UFH at activities > or = 20 
      I.U./ml. No significant systematic influence could be demonstrated for LMWH on 
      the aggregation induced by collagen as well as for LMWH and UFH on the platelet 
      aggregation induced by ADP. Capillary in vitro bleeding time (closure time) was 
      prolonged only after adding high concentrations of UFH (> or = 10 I.U./ml) and 
      LMWH (> or = 50 Anti-FXaU/ml) to the sample material. The results document the 
      unimportant influence of therapeutic levels of UFH and LMWH on platelet function 
      in dogs. Therefore, the remarkable inhibition of the aggregation induced by 
      thrombin reflects mainly the antithrombin effect.
FAU - Nimmerfall, K
AU  - Nimmerfall K
AD  - Klinik fur kleine Haustiere, Tierarztlichen Hochschule Hannover.
FAU - Mischke, R
AU  - Mischke R
LA  - ger
PT  - English Abstract
PT  - Journal Article
TT  - Einfluss von unfraktioniertem und niedermolekularem Heparin auf die 
      Thrombozytenaggregation und die In-vitro-Blutungszeit beim Hund.
PL  - Germany
TA  - Dtsch Tierarztl Wochenschr
JT  - DTW. Deutsche tierarztliche Wochenschrift
JID - 7706565
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - Bleeding Time/methods/*veterinary
MH  - Dogs
MH  - Heparin/*pharmacology
MH  - Heparin, Low-Molecular-Weight/*pharmacology
MH  - In Vitro Techniques
MH  - Platelet Aggregation/*drug effects
MH  - Reference Values
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
PST - ppublish
SO  - Dtsch Tierarztl Wochenschr. 1999 Oct;106(10):439-44.