PMID- 10589490
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 82
IP  - 6
DP  - 1999 Nov-Dec
TI  - Rapid immunoaffinity-based method for determination of zearalenone in corn by 
      fluorometry and liquid chromatography.
PG  - 1364-8
AB  - An immunoaffinity-based method was developed to determine zearalenone in corn. 
      Corn samples were extracted in acetonitrile-water (90 + 10, v/v), applied to an 
      immunoaffinity column, and eluted with methanol. The isolated toxin was 
      quantitated either by reaction with aluminum chloride hexahydrate (AlCl3.6H2O) 
      prior to measurement with a fluorometer or injection into a liquid 
      chromatographic (LC) system with a fluorescence detector. Performance was 
      evaluated in terms of antibody specificity, limit of detection, percentage 
      recovery, precision, column capacity, assay linearity, and comparison with AOAC 
      Official Method 985.18. With the immunoaffinity column cleanup procedure, only 
      zearalenone and its metabolites were recognized by the antibody (> or = 75% 
      recovery). Limits of detection were 0.10 microgram/g for the fluorometer and 0.10 
      or 0.0025 microgram/g (sensitive method) for the LC method. Percentage recovery 
      averaged 105% (fluorometer) and 93% (LC method), with average relative standard 
      deviations (RSDs) of 15.7 and 9.3%. Naturally contaminated samples gave 
      comparable RSDs of 8.3 and 9.9% for the fluorometer and LC methods, respectively. 
      Column capacity was 4.0 micrograms with 89% recovery. Assay linearity was 
      comparable for both methods (r2 = 0.998). Optimum assay ranges were 0.10-5.0 
      micrograms/g for the fluorometer and 0.10-50 or 0.0025-5.0 micrograms/g 
      (sensitive method) for the LC method. Comparative analysis of 17 naturally 
      contaminated corn samples using Zearala Test LC and the official AOAC LC method 
      for detection of zearalenone showed that Zearala Test is statistically comparable 
      to the AOAC Official Method 985.18 (r2 = 0.747).
FAU - Kruger, S C
AU  - Kruger SC
AD  - VICAM, Watertown, MA 02472, USA.
FAU - Kohn, B
AU  - Kohn B
FAU - Ramsey, C S
AU  - Ramsey CS
FAU - Prioli, R
AU  - Prioli R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 5W827M159J (Zearalenone)
SB  - IM
MH  - Antibody Specificity
MH  - *Chromatography, High Pressure Liquid
MH  - *Fluorometry
MH  - Immunoassay/*methods
MH  - Sensitivity and Specificity
MH  - Zea mays/*chemistry
MH  - Zearalenone/*analysis
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1999 Nov-Dec;82(6):1364-8.