PMID- 10589773
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20041117
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 5
IP  - 11
DP  - 1999 Nov
TI  - Deletion of chromosome 1p and loss of expression of alkaline phosphatase indicate 
      progression of meningiomas.
PG  - 3569-77
AB  - Meningiomas are cytogenetically characterized by loss of one chromosome 22 as a 
      typical primary aberration and progression-associated secondary chromosome 
      changes, of which monosomy 1p is the most common. The aim of this study was to 
      evaluate the significance of monosomy 1p and enzyme activity loss of tissue 
      nonspecific alkaline phosphatase (ALPL), whose gene maps to chromosome 
      1p36.1-p34, as parameters for the diagnosis of progression-prone meningiomas. We 
      analyzed smear preparations of 56 meningiomas and additional paraffin sections of 
      17 of the cases by two-color fluorescence in situ hybridization (FISH) using the 
      D1Z1 and D1Z2 probes and by a metaphase cytogenetic analysis of 30 of these 
      tumors. The results were compared to clinical and morphological parameters and 
      the expression of ALPL. Smear preparations showed deletion of 1p36 in 27% of 
      common-type, 70% of atypical (intermediate-type), and 100% of anaplastic 
      meningiomas. Monosomy 1p, as detected by FISH or the karyotype, was strongly 
      associated with complete loss of ALPL activity. Intermediate-type and anaplastic 
      meningiomas of younger patients displayed an increasing rate of cells with 
      trisomy 1q and relative loss of 1p. The highly significant correlation of FISH 
      results and ALPL histochemistry with clinical parameters gives evidence of their 
      strong prognostic relevance. The complete activity loss of ALPL and the 
      immunologically detected loss of ALPL protein in areas of meningiomas with 
      monosomy 1p indicate a cytogenetically undetectable inactivation of the 
      homologous Alpl allele. The apparently homozygous loss of expression of ALPL 
      supports the notion that Alpl is a candidate tumor suppressor gene in 
      meningiomas.
FAU - Muller, P
AU  - Muller P
AD  - Department of Human Genetics, Medical School of the University of the Saarland, 
      Homburg/Saar, Germany.
FAU - Henn, W
AU  - Henn W
FAU - Niedermayer, I
AU  - Niedermayer I
FAU - Ketter, R
AU  - Ketter R
FAU - Feiden, W
AU  - Feiden W
FAU - Steudel, W I
AU  - Steudel WI
FAU - Zang, K D
AU  - Zang KD
FAU - Steilen-Gimbel, H
AU  - Steilen-Gimbel H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/*genetics
MH  - Biopsy
MH  - *Chromosome Deletion
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 1
MH  - Disease Progression
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Meningeal Neoplasms/*enzymology/*genetics/pathology
MH  - Meningioma/enzymology/*genetics/pathology
MH  - *Monosomy
MH  - Trisomy
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
PST - ppublish
SO  - Clin Cancer Res. 1999 Nov;5(11):3569-77.