PMID- 10591554
OWN - NLM
STAT- MEDLINE
DCOM- 20000105
LR  - 20190710
IS  - 0022-3468 (Print)
IS  - 0022-3468 (Linking)
VI  - 34
IP  - 11
DP  - 1999 Nov
TI  - Highly sensitive analysis for N-myc amplification in neuroblastoma based on 
      fluorescence in situ hybridization.
PG  - 1615-9
AB  - BACKGROUND/PURPOSE: The N-myc amplification status in neuroblastoma has been 
      evaluated previously for the whole tumor by the Southern blot method. The aim of 
      this study is to evaluate the effectiveness of the fluorescence in situ 
      hybridization (FISH) method to analyze N-myc amplification in neuroblastoma and 
      compare the findings with those using the Southern blot method. METHODS: In 26 
      neuroblastoma primary tumors and metastatic lesions, the N-myc amplification 
      status was evaluated by both the Southern blot method and FISH method. RESULTS: 
      Of the 22 samples with no N-myc amplification using Southern blot, no cells with 
      N-myc amplification using FISH were present in 21 of the samples. However, one 
      metastatic liver lesion showed 16% of the nuclei to display more than 10 copies 
      of N-myc based on FISH analysis. In the 4 remaining samples with N-myc 
      amplification using the Southern blot method (17 copies, 15 copies, 6 copies, and 
      3 copies), the rates of cells with more than 10 copies of N-myc based on a FISH 
      analysis were 79%, 68%, 94%, and 9%, respectively. CONCLUSIONS: The FISH method 
      can detect more accurately N-myc amplification than the Southern blot method 
      either when the rate of cells with N-myc amplification is low or intratumor 
      heterogeneity is present.
FAU - Tajiri, T
AU  - Tajiri T
AD  - Department of Pediatric Surgery, Kyushu University, Fukuoka, Japan.
FAU - Shono, K
AU  - Shono K
FAU - Fujii, Y
AU  - Fujii Y
FAU - Noguchi, S
AU  - Noguchi S
FAU - Kinoshita, Y
AU  - Kinoshita Y
FAU - Tsuneyoshi, M
AU  - Tsuneyoshi M
FAU - Suita, S
AU  - Suita S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
SB  - IM
MH  - Blotting, Southern
MH  - Child, Preschool
MH  - Culture Techniques
MH  - Female
MH  - Gene Amplification
MH  - *Gene Expression Regulation, Neoplastic
MH  - *Genes, myc
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Male
MH  - Neuroblastoma/*genetics
MH  - Sensitivity and Specificity
EDAT- 1999/12/11 00:00
MHDA- 1999/12/11 00:01
CRDT- 1999/12/11 00:00
PHST- 1999/12/11 00:00 [pubmed]
PHST- 1999/12/11 00:01 [medline]
PHST- 1999/12/11 00:00 [entrez]
AID - S0022-3468(99)90628-0 [pii]
AID - 10.1016/s0022-3468(99)90628-0 [doi]
PST - ppublish
SO  - J Pediatr Surg. 1999 Nov;34(11):1615-9. doi: 10.1016/s0022-3468(99)90628-0.