PMID- 10591562
OWN - NLM
STAT- MEDLINE
DCOM- 20000105
LR  - 20190710
IS  - 0022-3468 (Print)
IS  - 0022-3468 (Linking)
VI  - 34
IP  - 11
DP  - 1999 Nov
TI  - Critical timing of bladder embryogenesis in an adriamycin-exposed rat fetal 
      model: a clue to the origin of the bladder.
PG  - 1647-51
AB  - BACKGROUND/PURPOSE: Administration of Adriamycin (ADR) in utero to pregnant rats 
      (vaginal plug, day 0) on gestational days (GD) 6 to 9 resulted in the offspring 
      having a cluster of malformations, including absence of bladder in 100% of cases. 
      This study aimed to determine the critical timing of the embryological window in 
      bladder development in this animal model. METHODS: Timed-pregnant rats were 
      divided randomly and injected intraperitoneally with ADR at 2 mg/kg on GD 6 to 9; 
      GD 7 to 10; GD 8 to 11; GD 9 to 12; GD 6,8, and 9 (missing GD 7); and GD 6, 7, 
      and 9 (missing GD 8). The control group received saline. Fetuses were harvested 
      near term on GD 21 and dissected under a dissecting microscope and examined for 
      gross anorectal and urogenital anomalies. RESULTS: Administration of ADR on GD 6 
      to 9 (n = 63); GD 7 to 10 (n = 42); and GD 6, 7, and 9 (n = 35) resulted in 100%, 
      83%, and 77% bladder agenesis respectively, in contrast with 53% and 26% on GD 8 
      to 11 (n = 36) and GD 6, 8, and 9 (n = 49), respectively. The control (n = 52) 
      and the GD 9 to 12 (n = 27) groups all had normal bladder development. The 
      proportion of other urogenital and anorectal anomalies mirror that of bladder 
      agenesis. CONCLUSION: The results showed GD 7 to be the critical embryological 
      timing in which bladder development can be affected by ADR, possibly by targeting 
      the gene that is expressed in the embryonic bladder during this narrow time 
      interval.
FAU - Liu, M I
AU  - Liu MI
AD  - F. Douglas Stephens Surgical Research Laboratory, Royal Children's Hospital 
      Research Institute, Melbourne, Australia.
FAU - Hutson, J M
AU  - Hutson JM
FAU - Zhou, B
AU  - Zhou B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Doxorubicin
MH  - Embryonic and Fetal Development/drug effects
MH  - Female
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reference Values
MH  - Time Factors
MH  - Urinary Bladder/*abnormalities/*embryology
MH  - Urogenital Abnormalities/chemically induced/*embryology
EDAT- 1999/12/11 00:00
MHDA- 1999/12/11 00:01
CRDT- 1999/12/11 00:00
PHST- 1999/12/11 00:00 [pubmed]
PHST- 1999/12/11 00:01 [medline]
PHST- 1999/12/11 00:00 [entrez]
AID - S0022-3468(99)90636-X [pii]
AID - 10.1016/s0022-3468(99)90636-x [doi]
PST - ppublish
SO  - J Pediatr Surg. 1999 Nov;34(11):1647-51. doi: 10.1016/s0022-3468(99)90636-x.