PMID- 10592049
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190921
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 435
IP  - 5
DP  - 1999 Nov
TI  - 20q13.2 amplification in intraductal hyperplasia adjacent to in situ and invasive 
      ductal carcinoma of the breast.
PG  - 469-72
AB  - The 20q13 region harboring recently described putative oncogenes is frequently 
      amplified in invasive ductal carcinoma (IDC). The aim of this study was to 
      examine the 20q13 copy number in intraduct hyperplasia (IH), atypical duct 
      hyperplasia (ADH), and ductal carcinoma in situ (DCIS) adjacent to IDC. In 5 
      patients, comparative genomic hybridization (CGH) after laser microdissection 
      revealed 20q13 amplification in four of five cases of IH, in all of three cases 
      of IH with atypia, all five of DCIS, and all five of IDC. Fluorescence in situ 
      hybridization (FISH) confirmed the amplification at 20q13.2 in IH in the two 
      specimens analyzed. The amplification rate, however, was higher in DCIS and IDC. 
      In phenotypically normal ductal epithelium normal values were found for 20q13 
      copy number by FISH (n=2) and CGH (n=5). Although the number of cases presented 
      here is small, our results suggest that mutations in the 20q13.2 region in IH may 
      be associated with accelerated proliferation and hyperplasia of the ductal 
      epithelium. Progression to DCIS and ICD is accompanied by a further increase in 
      the 20q13.2 copy number.
FAU - Werner, M
AU  - Werner M
AD  - Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische 
      Universitat Munchen, Klinikum rechts der Isar, Ismaninger Strasse 22, D-81675 
      Munchen, Germany. Martin.Werner@lrz.tum.de
FAU - Mattis, A
AU  - Mattis A
FAU - Aubele, M
AU  - Aubele M
FAU - Cummings, M
AU  - Cummings M
FAU - Zitzelsberger, H
AU  - Zitzelsberger H
FAU - Hutzler, P
AU  - Hutzler P
FAU - Hofler, H
AU  - Hofler H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - Carcinoma, Ductal, Breast/*genetics/pathology
MH  - Carcinoma, Intraductal, Noninfiltrating/*genetics/pathology
MH  - Centromere/genetics
MH  - Chromosomes, Human, Pair 20/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphatic Metastasis/genetics
MH  - Nucleic Acid Hybridization
EDAT- 1999/12/11 00:00
MHDA- 1999/12/11 00:01
CRDT- 1999/12/11 00:00
PHST- 1999/12/11 00:00 [pubmed]
PHST- 1999/12/11 00:01 [medline]
PHST- 1999/12/11 00:00 [entrez]
AID - 94350469.428 [pii]
AID - 10.1007/s004280050429 [doi]
PST - ppublish
SO  - Virchows Arch. 1999 Nov;435(5):469-72. doi: 10.1007/s004280050429.