PMID- 10594059
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20240421
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 24
DP  - 1999 Dec 15
TI  - Neuregulin induces GABA(A) receptor subunit expression and neurite outgrowth in 
      cerebellar granule cells.
PG  - 10757-66
AB  - Neuregulin (NRG), a growth and differentiation factor that signals via erbB 
      receptor tyrosine kinases, has been shown to have biological effects in both the 
      CNS and the peripheral nervous system. We report here that erbB4 is expressed in 
      mature cerebellar granule cells, where it appears to be concentrated at the 
      granule cell postsynaptic terminals. We also show that one form of NRG, Ig-NRG, 
      plays a crucial role in aspects of cerebellar granule cell development in vitro. 
      First, Ig-NRG treatment of granule cells in culture selectively induces the 
      expression of the GABA(A) receptor beta2 subunit. This increase in subunit 
      expression is paralleled by an increase in functional GABA(A) receptors. In 
      contrast to its effects on GABA(A) receptor subunit expression, Ig-NRG does not 
      upregulate NMDA receptor N2B and N2C subunit expression. Second, we demonstrate 
      that Ig-NRG also enhances neurite outgrowth from cultured granule cells. Ig-NRG 
      does not, however, act as a survival factor for the granule cells. We have 
      compared the effect of Ig-NRG with the effects of brain-derived neurotrophic 
      factor (BDNF), a neurotrophin that exerts specific effects on granule cells in 
      culture, and found that BDNF does not mimic the effects of Ig-NRG on GABA(A) 
      receptor subunit expression. Our results show that Ig-NRG has specific effects on 
      granule cell development and maturation and may regulate these processes in vivo.
FAU - Rieff, H I
AU  - Rieff HI
AD  - Division of Neuroscience, Department of Neurology, Children's Hospital and 
      Harvard Medical School, Harvard Medical School, Boston, Massachusetts 02115, USA.
FAU - Raetzman, L T
AU  - Raetzman LT
FAU - Sapp, D W
AU  - Sapp DW
FAU - Yeh, H H
AU  - Yeh HH
FAU - Siegel, R E
AU  - Siegel RE
FAU - Corfas, G
AU  - Corfas G
LA  - eng
GR  - R01 NS35884/NS/NINDS NIH HHS/United States
GR  - R01 NS035884/NS/NINDS NIH HHS/United States
GR  - P30 HD018655/HD/NICHD NIH HHS/United States
GR  - 632EY07110-05/EY/NEI NIH HHS/United States
GR  - T32 EY007110/EY/NEI NIH HHS/United States
GR  - R01 NS034317/NS/NINDS NIH HHS/United States
GR  - P30-HD18655/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Neuregulins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, GABA-A)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (Erbb4 protein, rat)
RN  - EC 2.7.10.1 (Receptor, ErbB-4)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Animals, Newborn/growth & development
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Cerebellum/cytology/metabolism/*physiology
MH  - ErbB Receptors/metabolism
MH  - Neuregulins/*pharmacology
MH  - Neurites/*drug effects/*physiology
MH  - Neurons/drug effects/metabolism/*physiology
MH  - Protein Isoforms/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, ErbB-4
MH  - Receptors, GABA-A/drug effects/*metabolism
MH  - Synapses/metabolism
MH  - Up-Regulation
PMC - PMC6784934
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
PMCR- 2000/06/15
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
PHST- 2000/06/15 00:00 [pmc-release]
AID - 3721 [pii]
AID - 10.1523/JNEUROSCI.19-24-10757.1999 [doi]
PST - ppublish
SO  - J Neurosci. 1999 Dec 15;19(24):10757-66. doi: 10.1523/JNEUROSCI.19-24-10757.1999.