PMID- 10594296
OWN - NLM
STAT- MEDLINE
DCOM- 20000124
LR  - 20061115
IS  - 1046-199X (Print)
IS  - 1046-199X (Linking)
VI  - 10
IP  - 4
DP  - 1999 Dec
TI  - Identification of metal allergens in the local lymph node assay.
PG  - 207-12
AB  - BACKGROUND: The murine local lymph node assay (LLNA) has recently been endorsed 
      as a validated alternative to guinea pig methods for the identification of skin 
      sensitization hazard. Nevertheless, there has been some debate regarding the 
      utility of this method for the detection of metal contact allergens. OBJECTIVE: 
      In these investigations, we have used the LLNA to determine the skin 
      sensitization potential of 13 metal salts, 8 of which were considered to possess 
      a significant ability to sensitize man, whereas the remaining 5 were judged to 
      lack such potential. RESULTS: The predictions from the LLNA were correct for 7 of 
      the 8 (88%) sensitizing metals and for 4 of the 5 (80%) nonsensitizers when 
      considered against the experience of these metals as human skin sensitizers. 
      Thus, the overall predictive accuracy of the LLNA in relation to metals was 11/13 
      (85%), which is very similar to the accuracy of approximately 88% in relation to 
      a much larger number of low-molecular-weight organic chemicals, as reported 
      previously. CONCLUSION: These data provide support for the potential utility of 
      the LLNA in hazard identification of metal contact allergens.
FAU - Basketter, D A
AU  - Basketter DA
AD  - Unilever Safety and Environmental Assurance Centre, Toxicology Unit, Colworth 
      House, Zeneca Central Toxicology Laboratory, Alderley Park, Sharnbrook, 
      Bedfordshire, UK.
FAU - Lea, L J
AU  - Lea LJ
FAU - Cooper, K J
AU  - Cooper KJ
FAU - Ryan, C A
AU  - Ryan CA
FAU - Gerberick, G F
AU  - Gerberick GF
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Canada
TA  - Am J Contact Dermat
JT  - American journal of contact dermatitis : official journal of the American Contact 
      Dermatitis Society
JID - 9100472
RN  - 0 (Allergens)
RN  - 0 (Metals)
SB  - IM
MH  - Allergens/*analysis/immunology
MH  - Animals
MH  - Culture Techniques
MH  - Dermatitis, Allergic Contact/*diagnosis/immunology/prevention & control
MH  - Female
MH  - Humans
MH  - Lymph Nodes/*chemistry/immunology
MH  - Male
MH  - Metals/*analysis/immunology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Patch Tests
MH  - Predictive Value of Tests
MH  - Sensitivity and Specificity
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - S1046199X99000299 [pii]
AID - 10.1053/AJCD01000207 [doi]
PST - ppublish
SO  - Am J Contact Dermat. 1999 Dec;10(4):207-12. doi: 10.1053/AJCD01000207.