PMID- 10594350
OWN - NLM
STAT- MEDLINE
DCOM- 20000107
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 230
IP  - 1-2
DP  - 1999 Nov 19
TI  - A fluorimeter-based RT-PCR method for the detection and quantitation of porcine 
      cytokines.
PG  - 19-27
AB  - In this paper, we describe a fluorimeter-based, closed-tube, polymerase chain 
      reaction (PCR) assay for the detection and quantification of the mRNA of porcine 
      interleukin 1alpha (IL1alpha) and interleukin 2 (IL2) cytokines in peripheral 
      blood leukocytes (PBLs) using melting curve analysis and compare it to a standard 
      PCR performed in a block-based thermocycler.
FAU - Spagnuolo-Weaver, M
AU  - Spagnuolo-Weaver M
AD  - Department of Virology, Veterinary Sciences Division, Stormont, Belfast, UK. 
      m.spagnuolo@qub.a.cuk
FAU - Fuerst, R
AU  - Fuerst R
FAU - Campbell, S T
AU  - Campbell ST
FAU - Meehan, B M
AU  - Meehan BM
FAU - McNeilly, F
AU  - McNeilly F
FAU - Adair, B
AU  - Adair B
FAU - Allan, G
AU  - Allan G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Actins)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Actins/genetics
MH  - Animals
MH  - Cytokines/*analysis/*genetics
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Evaluation Studies as Topic
MH  - Fluorometry/*methods
MH  - Gene Expression
MH  - Interleukin-1/analysis/genetics
MH  - Interleukin-2/analysis/genetics
MH  - Leukocytes/immunology
MH  - Plasmids/genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Swine
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - S0022-1759(99)00114-3 [pii]
AID - 10.1016/s0022-1759(99)00114-3 [doi]
PST - ppublish
SO  - J Immunol Methods. 1999 Nov 19;230(1-2):19-27. doi: 
      10.1016/s0022-1759(99)00114-3.