PMID- 10595737
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20190513
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 90
IP  - 10
DP  - 1999 Oct
TI  - Characterization of the MEN1 gene product, menin, by site-specific polyclonal 
      antibodies.
PG  - 1088-95
AB  - The gene associated with multiple endocrine neoplasia type 1 (MEN 1), designated 
      MEN1, has recently been identified. This gene shows no homology to other known 
      genes, and its expression is not restricted to endocrine organs as estimated by 
      northern blotting. Expression of the MEN1 gene product, menin, has been studied 
      only in a few tissues. In this report, expression of menin in various cells and 
      mouse tissues was studied using two polyclonal antibodies against menin. 
      Expression of menin as a 76 kDa single protein was observed in all cell lines 
      examined, regardless of origin. Two nuclear localization signals of the menin 
      have been reported, but through the study of mutant menin in lymphocytes from 
      subjects with MEN 1, impaired nuclear localization of the mutant menin was 
      observed even though the mutant retained one of the two nuclear localization 
      signals (NLSs). Menin was stable in vitro with a half-life of over 24 h at 37 
      degrees C. In the cell, the half-life of wild-type menin was about 10 h, while 
      that of the mutant was about 2 h. The mutant rapidly disappeared from the 
      nucleus.
FAU - Ikeo, Y
AU  - Ikeo Y
AD  - Department of Aging Medicine and Geriatrics, Shinshu University School of 
      Medicine, Asahi, Matsumoto.
FAU - Sakurai, A
AU  - Sakurai A
FAU - Hashizume, K
AU  - Hashizume K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Cell Line
MH  - *Genes, Tumor Suppressor
MH  - HL-60 Cells
MH  - Humans
MH  - Lymphocytes/pathology
MH  - Mice
MH  - Multiple Endocrine Neoplasia Type 1/blood/*genetics
MH  - Neoplasm Proteins/*analysis/*genetics
MH  - Organ Specificity
MH  - PC12 Cells
MH  - Protein Biosynthesis
MH  - *Proto-Oncogene Proteins
MH  - Rats
MH  - Recombinant Proteins/analysis/biosynthesis
MH  - Transfection
MH  - Tumor Cells, Cultured
PMC - PMC5925994
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
PMCR- 1999/10/01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
PHST- 1999/10/01 00:00 [pmc-release]
AID - S0910505099801732 [pii]
AID - CAE1088 [pii]
AID - 10.1111/j.1349-7006.1999.tb00682.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 1999 Oct;90(10):1088-95. doi: 
      10.1111/j.1349-7006.1999.tb00682.x.