PMID- 10595847
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 23
IP  - 7
DP  - 1999 Nov-Dec
TI  - A gas chromatography-mass spectrometry method for the quantitation of 
      clobenzorex.
PG  - 603-8
AB  - Drugs metabolized to amphetamine or methamphetamine are potentially significant 
      concerns in the interpretation of amphetamine-positive urine drug-testing 
      results. One of these compounds, clobenzorex, is an anorectic drug that is 
      available in many countries. Clobenzorex (2-chlorobenzylamphetamine) is 
      metabolized to amphetamine by the body and excreted in the urine. Following 
      administration, the parent compound was detectable for a shorter time than the 
      metabolite amphetamine, which could be detected for days. Because of the 
      potential complication posed to the interpretation of amphetamin-positive drug 
      tests following administration of this drug, the viability of a current 
      amphetamine procedure using liquid-liquid extraction and conversion to the 
      heptafluorobutyryl derivative followed by gas chromatography-mass spectrometry 
      (GC-MS) analysis was evaluated for identification and quantitation of 
      clobenzorex. Qualitative identification of the drug was relatively 
      straightforward. Quantitative analysis proved to be a far more challenging 
      process. Several compounds were evaluated for use as the internal standard in 
      this method, including methamphetamine-d11, fenfluramine, benzphetamine, and 
      diphenylamine. Results using these compounds proved to be less than satisfactory 
      because of poor reproducibility of the quantitative values. Because of its 
      similar chromatographic properties to the parent drug, the compound 
      3-chlorobenzylamphetamine (3-Cl-clobenzorex) was evaluated in this study as the 
      internal standard for the quantitation of clobenzorex. Precision studies showed 
      3-Cl-clobenzorex to produce accurate and reliable quantitative results 
      (within-run relative standard deviations [RSDs] < 6.1%, between-run RSDs < 6.0%). 
      The limits of detection and quantitation for this assay were determined to be 1 
      ng/mL for clobenzorex.
FAU - Cody, J T
AU  - Cody JT
AD  - Clinical Research Squadron, Wilford Hall Medical Center, Lackland AFB, Texas 
      78236-5319, USA.
FAU - Valtier, S
AU  - Valtier S
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 0 (Appetite Depressants)
RN  - 4A5352XI2A (clobenzorex)
SB  - IM
MH  - Amphetamines/chemistry/*urine
MH  - Appetite Depressants/*analysis
MH  - Gas Chromatography-Mass Spectrometry/instrumentation/*methods
MH  - Humans
MH  - Reproducibility of Results
MH  - Substance Abuse Detection/methods
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - 10.1093/jat/23.7.603 [doi]
PST - ppublish
SO  - J Anal Toxicol. 1999 Nov-Dec;23(7):603-8. doi: 10.1093/jat/23.7.603.