PMID- 10596410
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20061115
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 30
IP  - 6
DP  - 1999 Nov-Dec
TI  - Effect of challenge dose and route on porcine reproductive and respiratory 
      syndrome virus (PRRSV) infection in young swine.
PG  - 629-38
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) is perceived to be 
      highly infectious because of the rapid spread of the virus through populations of 
      domestic swine throughout the world. However, no information has been published 
      on the minimum infectious dose of PRRSV and the effect of challenge dose on 
      clinical response. In this experiment, ten groups of pigs (n = 3 per group) were 
      inoculated with one of five different quantities (10(1)-10(5) fluorescent foci 
      units per millilitre) of PRRSV (isolate ISU-P) by either intramuscular or 
      intranasal routes. Clinical signs and body temperature were monitored for 21 
      days. Serum was collected periodically throughout the study period to monitor the 
      presence of virus in serum and the early immune response of pigs. A 2-mL inoculum 
      containing 10(1) fluorescent foci units of virus per millilitre was found 
      sufficient to achieve infection by either route. Time to onset of clinical signs 
      was highly associated with challenge dose (P < 0.01), regardless of route of 
      exposure. However, no dose- or route-dependent differences in the severity of 
      clinical manifestation were observed. No significant differences in the time of 
      onset or degree of humoural immune response to PRRSV infection were observed 
      between different treatment groups. However, intramuscular exposure appeared to 
      induce a more uniform antibody response compared to intranasal exposure. These 
      results confirmed that PRRSV is highly infectious; a fact that should be taken 
      into consideration when designing strategies for the prevention and control of 
      PRRSV.
FAU - Yoon, K J
AU  - Yoon KJ
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames 50011, USA. kyoon@iastate.edu
FAU - Zimmerman, J J
AU  - Zimmerman JJ
FAU - Chang, C C
AU  - Chang CC
FAU - Cancel-Tirado, S
AU  - Cancel-Tirado S
FAU - Harmon, K M
AU  - Harmon KM
FAU - McGinley, M J
AU  - McGinley MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Polymerase Chain Reaction
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity
MH  - Swine
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
PST - ppublish
SO  - Vet Res. 1999 Nov-Dec;30(6):629-38.