PMID- 10598141
OWN - NLM
STAT- MEDLINE
DCOM- 20000105
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 115
IP  - 2
DP  - 1999 Dec
TI  - Hemizygous deletions of chromosome band 16q24 in Wilms tumor: detection by 
      fluorescence in situ hybridization.
PG  - 100-5
AB  - Loss of heterozygosity (LOH) for markers on chromosome arm 16q in Wilms tumor has 
      been linked to an increased risk of treatment failure. We therefore postulated 
      that fluorescence in situ hybridization (FISH) with probes from this region might 
      enhance current strategies for identifying high-risk patients at diagnosis. In a 
      blinded comparative pilot study of 19 Wilms tumor samples from 18 patients with 
      favorable histology, FISH and DNA polymorphism analysis yielded concordant 
      results in 14 cases, either retention (n = 6) or loss (n = 8) of chromosome arm 
      16q markers. Discordant findings in 4 of the 5 remaining cases resulted from 
      detection of LOH, but no loss by FISH. Two of these cases, directly comparable at 
      marker D16S422, appeared to have tumor-specific uniparental disomy, in that 2 
      copies of D16S422 and the 16 centromere were evident, despite LOH. In 2 other 
      cases, the discrepancies could be explained by LOH confined to loci distal to the 
      D16S422 locus. In the fifth case, FISH detected 2 distinct populations of tumor 
      cells, one characterized by normal diploidy and the other by monosomy 16, whereas 
      DNA polymorphism analysis failed to indicate LOH altogether. Thus, FISH confirmed 
      the presence of allelic loss (hence, the possible location of biologically 
      important tumor suppressor genes) on the distal long arm of chromosome 16 in 
      cases of favorable-histology Wilms tumor, with the advantages of technical 
      simplicity, successful analysis of samples that were otherwise uninformative by 
      analysis of DNA polymorphisms, and the addition of internal controls for 
      chromosomal aneusomy. We suggest that combined analysis of the chromosome 16q 
      region in Wilms tumor by FISH and DNA polymorphism analysis would improve 
      evaluations to identify high-risk patients who might benefit from alternative 
      therapy.
FAU - Shearer, P D
AU  - Shearer PD
AD  - Department of Hematology/Oncology, St. Jude Children's Research Hospital, 
      Memphis, Tennessee 38105, USA.
FAU - Valentine, M B
AU  - Valentine MB
FAU - Grundy, P
AU  - Grundy P
FAU - DeCou, J M
AU  - DeCou JM
FAU - Banavali, S D
AU  - Banavali SD
FAU - Komuro, H
AU  - Komuro H
FAU - Green, D M
AU  - Green DM
FAU - Beckwith, J B
AU  - Beckwith JB
FAU - Look, A T
AU  - Look AT
LA  - eng
GR  - CA21765/CA/NCI NIH HHS/United States
GR  - CA71907/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 16
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Homozygote
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Kidney Neoplasms/*genetics
MH  - Loss of Heterozygosity
MH  - Male
MH  - Polymorphism, Genetic
MH  - Wilms Tumor/*genetics
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - S016546089900093X [pii]
AID - 10.1016/s0165-4608(99)00093-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Dec;115(2):100-5. doi: 
      10.1016/s0165-4608(99)00093-x.