PMID- 10598867
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190831
IS  - 0165-0270 (Print)
IS  - 0165-0270 (Linking)
VI  - 93
IP  - 1
DP  - 1999 Oct 30
TI  - A rapid method for determination of cell survival in primary neuronal DRG 
      cultures.
PG  - 81-9
AB  - A simple and rapid enzyme-linked immunosorbent assay (ELISA) has been developed 
      to provide an alternative to cell counting to detect increases in cell survival 
      in primary neuronal cultures. This sensitive assay has the advantage of being 
      less time consuming and labour intensive than cell counting, can be used to 
      quantify cell survival and is more accurate than estimation methods of counting. 
      The ELISA uses an antibody raised to GAP-43, a growth-associated protein which is 
      strongly expressed by developing and regenerating neurones. The effects of nerve 
      growth factor (NGF), neurotrophin-3 (NT-3) and brain-derived neurotrophic factor 
      (BDNF) on GAP-43 immunoreactivity in dissociated primary cultures of rat and 
      chick dorsal root ganglia have been compared to results obtained by cell 
      counting. Data show that human NGF produced the greatest increase in 
      GAP-43-immunoreactive neurones in both species; this increase in immunoreactivity 
      correlated well with the increased survival shown by cell count data. Results 
      prove that the ELISA can also be used to accurately detect small changes in cell 
      survival as seen with NT-3 and BDNF, or potentiation of the effects obtained with 
      the trophic factor NT-3. In conclusion, this ELISA may be a useful tool to detect 
      neurotrophic effects of unknown agents or novel neurotrophins.
FAU - Young, L
AU  - Young L
AD  - Department of Pharmacology, Merck, Sharp and Dohme Laboratories, Neuroscience 
      Research Centre, Harlow, Essex, UK.
FAU - Bilsland, J
AU  - Bilsland J
FAU - Harper, S
AU  - Harper S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
RN  - 0 (GAP-43 Protein)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects/physiology
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Female
MH  - GAP-43 Protein/*metabolism
MH  - Ganglia, Spinal/*metabolism
MH  - Humans
MH  - Nerve Growth Factors/metabolism/pharmacology
MH  - Neurons/*metabolism
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - S0165-0270(99)00134-X [pii]
AID - 10.1016/s0165-0270(99)00134-x [doi]
PST - ppublish
SO  - J Neurosci Methods. 1999 Oct 30;93(1):81-9. doi: 10.1016/s0165-0270(99)00134-x.