PMID- 10599596
OWN - NLM
STAT- MEDLINE
DCOM- 20000120
LR  - 20071114
IS  - 1386-0291 (Print)
IS  - 1386-0291 (Linking)
VI  - 21
IP  - 2
DP  - 1999
TI  - Rate of deoxygenation modulates rheologic behavior of sickle red blood cells at a 
      given mean corpuscular hemoglobin concentration.
PG  - 125-35
AB  - Although the mean corpuscular hemoglobin concentration (MCHC) plays a dominant 
      role in the rheologic behavior of deoxygenated density-defined sickle red blood 
      cells (SS RBCs), previous studies have not explored the relationship between the 
      rate of deoxygenation and the bulk viscosity of SS RBCs at a given MCHC. In the 
      present study, we have subjected density-defined SS classes (i.e., medium-density 
      SS4 and dense SS5 discocytes) to varying deoxygenation rates. This approach has 
      allowed us to minimize the effects of SS RBC heterogeneity and investigate the 
      effect of deoxygenation rates at a given MCHC. The results show that the 
      percentages of granular cells, classic sickle cells and holly leaf forms in 
      deoxygenated samples are significantly influenced by the rate of deoxygenation 
      and the MCHC of a given discocyte subpopulation. Increasing the deoxygenation 
      rate using high K+ medium (pH 6.8), results in a greater percentage of granular 
      cells in SS4 suspensions, accompanied by a pronounced increase in the bulk 
      viscosity of these cells compared with gradually deoxygenated samples (mainly 
      classic sickle cells and holly leaf forms). The effect of MCHC becomes apparent 
      when SS5 dense cells are subjected to varying deoxygenation rates. At a given 
      deoxygenation rate, SS5 dense discocytes show a greater increase in the 
      percentage of granular cells than that observed for SS4 RBCs. Also, at a given 
      deoxygenation rate, SS5 suspensions exhibit a higher viscosity than SS4 
      suspensions with fast deoxygenation resulting in maximal increase in viscosity. 
      Although MCHC is the main determinant of SS RBC rheologic behavior, these studies 
      demonstrate for the first time that at a given MCHC, the rate of deoxygenation 
      (hence HbS polymerization rates) further modulates the rheologic behavior of SS 
      RBCs. Thus, both MCHC and the deoxygenation rate may contribute to 
      microcirculatory flow behavior of SS RBCs.
FAU - Kaul, D K
AU  - Kaul DK
AD  - Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, 
      USA. kaul@aecom.yu.edu
FAU - Liu, X D
AU  - Liu XD
LA  - eng
GR  - HL38655/HL/NHLBI NIH HHS/United States
GR  - HL45931/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Clin Hemorheol Microcirc
JT  - Clinical hemorheology and microcirculation
JID - 9709206
RN  - 0 (Biopolymers)
RN  - 0 (Hemoglobin, Sickle)
RN  - 0 (Hemoglobins)
RN  - 9008-02-0 (deoxyhemoglobin)
SB  - IM
MH  - Anemia, Sickle Cell/*blood
MH  - Biopolymers
MH  - Blood Viscosity
MH  - Erythrocytes, Abnormal/classification/*metabolism/pathology
MH  - Hemoglobin, Sickle/*metabolism/ultrastructure
MH  - Hemoglobins/*analysis/*metabolism
MH  - Humans
MH  - Microcirculation
MH  - Microscopy, Electron
MH  - Microscopy, Electron, Scanning
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
PST - ppublish
SO  - Clin Hemorheol Microcirc. 1999;21(2):125-35.