PMID- 10600795
OWN - NLM
STAT- MEDLINE
DCOM- 20000120
LR  - 20180920
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 277
IP  - 6
DP  - 1999 Dec
TI  - Effect of short-term exercise training on insulin-stimulated PI 3-kinase activity 
      in human skeletal muscle.
PG  - E1055-60
LID - 10.1152/ajpendo.1999.277.6.E1055 [doi]
AB  - The purpose of this study was to determine if the improvement in insulin 
      sensitivity with exercise training is associated with enhanced 
      phosphatidylinositol 3-kinase (PI 3-kinase) activity. Nine sedentary men were 
      studied before and after 7 days of exercise training (1 h/day, approximately 75% 
      maximal oxygen consumption). Insulin sensitivity was determined with a 
      euglycemic-hyperinsulinemic glucose clamp in the sedentary state and 15-17 h 
      after the final exercise bout. PI 3-kinase activity was determined from samples 
      (vastus lateralis) obtained in the fasted condition and after 60 min of 
      submaximal insulin stimulation during the clamp. After exercise, glucose infusion 
      rate increased (P < 0. 05) significantly (means +/- SE, 7.8 +/- 0.5 vs. 9.8 +/- 
      0.8 mg. kg(-1). min(-1)), indicating improved insulin sensitivity. 
      Insulin-stimulated (insulin stimulated/fasting) phosphotyrosine 
      immunoprecipitable PI 3-kinase activity also increased significantly (P < 0.05) 
      with exercise (3.1 +/- 0.8-fold) compared with the sedentary condition (1.3 +/- 
      0.1-fold). There was no change in fasting PI 3-kinase activity. These data 
      suggest that an enhancement of insulin signal transduction in skeletal muscle may 
      contribute to the improvement in insulin action with exercise.
FAU - Houmard, J A
AU  - Houmard JA
AD  - Human Performance Laboratory and Department of Exercise and Sport Science, East 
      Carolina University, Greenville, North Carolina 27858, USA. houmardj@mail.ecu.edu
FAU - Shaw, C D
AU  - Shaw CD
FAU - Hickey, M S
AU  - Hickey MS
FAU - Tanner, C J
AU  - Tanner CJ
LA  - eng
GR  - AG-100-25/AG/NIA NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adult
MH  - Blood Glucose
MH  - Body Mass Index
MH  - Exercise/*physiology
MH  - Glucose/administration & dosage
MH  - Humans
MH  - Hyperinsulinism/metabolism
MH  - Insulin/*blood
MH  - Insulin Resistance
MH  - Male
MH  - Muscle Contraction/physiology
MH  - Muscle, Skeletal/*enzymology
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Signal Transduction/physiology
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1152/ajpendo.1999.277.6.E1055 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Dec;277(6):E1055-60. doi: 10.1152/ajpendo.1999.277.6.E1055.