PMID- 10601423
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20171213
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 82
IP  - 6
DP  - 1999 Dec
TI  - Voltage-sensitive calcium currents are acutely increased by nerve growth factor 
      in PC12 cells.
PG  - 2847-52
AB  - Whole cell calcium currents were recorded from PC12 cells with the perforated 
      patch technique. Currents were evoked by step depolarization from a holding 
      potential of -90 mV. Nerve growth factor (NGF) increased calcium currents through 
      L-type calcium channels by >75% within 3-5 min. This increase was inhibited by 
      K-252a, by nifedipine, and by inhibition or down-regulation of kinase C. 
      Brain-derived neurotrophic factor (BDNF) also increased calcium current, but to a 
      smaller extent. Thus increases in calcium current can be linked to activation of 
      either the high- or the low-affinity nerve growth factor receptor. Increases in 
      presynaptic calcium uptake appear to be a crucial element in the short-term 
      actions of the neurotrophins on neurotransmitter release leading to long-term 
      potentiation. Also, the control of calcium uptake is likely to be an important 
      factor in the long-term actions of the neurotrophins on neuronal survival and 
      neuronal protection. The present data indicate that the PC12 cell may be a useful 
      model for studying the effect of the neurotrophins on calcium uptake.
FAU - Jia, M
AU  - Jia M
AD  - Laboratory of Developmental Neurobiology, National Institute of Child Health and 
      Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
FAU - Li, M
AU  - Li M
FAU - Liu, X W
AU  - Liu XW
FAU - Jiang, H
AU  - Jiang H
FAU - Nelson, P G
AU  - Nelson PG
FAU - Guroff, G
AU  - Guroff G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium Channels, L-Type)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Calcium/metabolism
MH  - Calcium Channels, L-Type/*drug effects
MH  - Long-Term Potentiation/physiology
MH  - Nerve Growth Factors/metabolism/*pharmacology
MH  - PC12 Cells
MH  - Protein Kinase C/metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synapses/physiology
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1152/jn.1999.82.6.2847 [doi]
PST - ppublish
SO  - J Neurophysiol. 1999 Dec;82(6):2847-52. doi: 10.1152/jn.1999.82.6.2847.