PMID- 10601554
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190516
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 16
IP  - 1
DP  - 2000 Jan
TI  - Cloning of a human hepatocyte growth factor/scatter factor transcription variant 
      from a gastric cancer cell line HSC-39.
PG  - 105-8
AB  - A new transcription variant of hepatocyte growth factor/scatter factor (HGF/SF) 
      was cloned from human gastric cancer cell line HSC-39. Northern blot analysis of 
      eight human gastric cancer cell lines (TMK-1, MKN-1, MKN-7, MKN-28, MKN-45, 
      MKN-74, KATO-III and HSC-39) demonstrated that HSC-39 cells expressed a 1.3 kb 
      abnormal HGF/SF transcript. Screening of 1 x 10(6) colonies of cDNA library from 
      HSC-39 constructed in pAP3neo mammalian expression vector selected four positive 
      clones containing HGF/SF transcript. Among them, two contained a 1.3 kbp insert 
      detecting the identical transcript to that obtained with HGF/SF probe by Northern 
      blotting. Deoxynucleotide sequencing of the 1.3 kbp insert revealed that it was 
      composed of a part of HGF/SF cDNA from exon 14 to exon 18, corresponding to the 
      whole sequence of HGF/SF light chain, with 5' 75 nucleotides unrelated to any 
      sequence involved in HGF/SF.
FAU - Yokozaki, H
AU  - Yokozaki H
AD  - First Department of Pathology, Hiroshima University School of Medicine, 
      Minami-ku, Hiroshima 734-8551, Japan.
FAU - Tahara, H
AU  - Tahara H
FAU - Oue, N
AU  - Oue N
FAU - Tahara, E
AU  - Tahara E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (DNA, Complementary)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cloning, Organism
MH  - DNA, Complementary/analysis
MH  - Gene Library
MH  - *Genetic Variation
MH  - Hepatocyte Growth Factor/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Stomach Neoplasms/*genetics
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.3892/ijo.16.1.105 [doi]
PST - ppublish
SO  - Int J Oncol. 2000 Jan;16(1):105-8. doi: 10.3892/ijo.16.1.105.