PMID- 10602006
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20180420
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 12
DP  - 1999 Dec
TI  - CD8(+) T cell cytolytic activity independent of mitogen-activated protein kinase 
      / extracellular regulatory kinase signaling (MAP kinase / ERK).
PG  - 3971-7
AB  - Cytotoxicity is a major effector function of CD8(+) T cells. Although 
      mitogen-activated protein kinase (MAP kinase) / extracellular regulatory kinase 
      (ERK) activity is indispensable for cytotoxic activity of most CD8(+) T cells a 
      portion of CD8(+) T cells appears resistant to MEK inhibition as cytotoxicity of 
      bulk cultures was partially preserved in the presence of a MEK inhibitor. We have 
      also identified a long-term CD8(+) T cell line with unaltered cytolytic activity 
      after prevention of ERK activation. Antigen-induced microtubule organizing center 
      (MTOC) reorientation was not prevented in this CD8(+) cell line by MEK 
      inhibition, in sharp contrast to the MTOC reorientation prevention in a CD8(+) T 
      cell clone with MEK inhibition-sensitive cytolytic activity. These findings 
      suggest that resistance of lysis to MEK inhibition may be due to a lack of ERK 
      control over MTOC reorientation in some CD8(+) T cells. Thus, there appears to be 
      a heterogeneity of ERK-regulated cytolytic activity in CD8(+) T cells, most 
      likely resulting from a differential control of ERK over MTOC motility.
FAU - Lilic, M
AU  - Lilic M
AD  - Michael Heidelberger Division of Immunology Department of Pathology and Kaplan 
      Comprehensive Cancer Center, NYU Medical Center, New York, USA.
FAU - Kulig, K
AU  - Kulig K
FAU - Messaoudi, I
AU  - Messaoudi I
FAU - Remus, K
AU  - Remus K
FAU - Jankovic, M
AU  - Jankovic M
FAU - Nikolic-Zugic, J
AU  - Nikolic-Zugic J
FAU - Vukmanovic, S
AU  - Vukmanovic S
LA  - eng
GR  - AI041573/AI/NIAID NIH HHS/United States
GR  - CA-02853/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (CD8 Antigens)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - CD8 Antigens/immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - *Cytotoxicity, Immunologic
MH  - MAP Kinase Signaling System/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinases/*immunology
MH  - Signal Transduction/*immunology
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1002/(SICI)1521-4141(199912)29:12<3971::AID-IMMU3971>3.0.CO;2-5 [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Dec;29(12):3971-7. doi: 
      10.1002/(SICI)1521-4141(199912)29:12<3971::AID-IMMU3971>3.0.CO;2-5.