PMID- 10602090
OWN - NLM
STAT- MEDLINE
DCOM- 20000215
LR  - 20220330
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 416
IP  - 3
DP  - 2000 Jan 17
TI  - Neurotrophin expression by spinal motoneurons in adult and developing rats.
PG  - 309-18
AB  - Expression of the neurotrophins NT-4, brain-derived neurotrophic factor (BDNF), 
      and NT-3 in adult rat lumbosacral spinal cord motoneurons is reported. A 
      sensitive in situ hybridization procedure demonstrates localization of the mRNA 
      for each of these neurotrophins within spinal motoneurons of the adult and in 
      early postnatal development. A majority of adult rat spinal cord lumbar 
      motoneurons (approximately 63%) express NT-4 mRNA as assessed by counting 
      motoneurons in the L4 and L5 segments of two adult rat spinal cords on adjacent 
      cresyl violet-stained and in situ hybridization sections. Similarly, a majority 
      of lumbar motoneurons (approximately 73%) express BDNF mRNA. Further analyses of 
      adjacent lumbar spinal cord sections revealed that many, although not all 
      motoneurons coexpress both NT-4 and BDNF mRNAs. At birth, the mRNA encoding NT-3 
      is expressed in motoneurons, but BDNF mRNA is not apparent until postnatal day 5 
      (P5) and NT-4 mRNA first appears at P9. The potential biological significance of 
      neurotrophin mRNA expression in spinal motoneurons is supported by 
      immunohistochemical localization of each neurotrophin protein in adult 
      motoneurons. We discuss the potential role of spinal cord neurotrophins as 
      autocrine or paracrine factors involved in modulating motoneuron synaptic 
      function.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Buck, C R
AU  - Buck CR
AD  - Department of Physiology, Emory University School of Medicine, Atlanta, Georgia 
      30322, USA. cbuck@emory.edu
FAU - Seburn, K L
AU  - Seburn KL
FAU - Cope, T C
AU  - Cope TC
LA  - eng
GR  - 1P01 32571/PHS HHS/United States
GR  - R01 NS38693/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Lumbar Vertebrae/anatomy & histology
MH  - Motor Neurons/*cytology/*metabolism
MH  - Nerve Growth Factors/*genetics
MH  - Neurotrophin 3/genetics
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sacrum/anatomy & histology
MH  - Spinal Cord/*cytology/*metabolism
EDAT- 1999/12/22 09:00
MHDA- 2000/02/19 09:00
CRDT- 1999/12/22 09:00
PHST- 1999/12/22 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 1999/12/22 09:00 [entrez]
AID - 10.1002/(SICI)1096-9861(20000117)416:3<309::AID-CNE3>3.0.CO;2-U [pii]
AID - 10.1002/(sici)1096-9861(20000117)416:3<309::aid-cne3>3.0.co;2-u [doi]
PST - ppublish
SO  - J Comp Neurol. 2000 Jan 17;416(3):309-18. doi: 
      10.1002/(sici)1096-9861(20000117)416:3<309::aid-cne3>3.0.co;2-u.