PMID- 10602421
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 13
IP  - 12
DP  - 1999 Dec
TI  - Investigation of clonal involvement of myeloid cells in Philadelphia-positive and 
      high hyperdiploid acute lymphoblastic leukemia.
PG  - 2000-6
AB  - Acute lymphoblastic leukemia (ALL) with a high hyperdiploid clone has a good 
      prognosis for both childhood and adult patients while patients with 
      Philadelphia-positive (Ph) ALL do badly at all age groups. It has been suggested 
      that different responses to treatment might be related to the cell of origin of 
      the leukemia with 'stem cell' cases responding less well than those arising in a 
      lymphoid committed progenitor cell. The clonal involvement of different cell 
      lineages in 12 patients with acute lymphoblastic leukemia has been examined by 
      applying fluorescence in situ hybridization (FISH) to detect chromosomal 
      abnormalities in bone marrow cells previously identified by morphology and/or 
      immunology. The karyotype of the malignant clone was either high hyperdiploid or 
      Philadelphia translocation (Ph) positive with a breakpoint in the minor 
      breakpoint cluster region of the BCRgene (m-BCR) or in the major breakpoint 
      cluster region of the BCR gene (M-BCR). The high hyperdiploid clone, in each 
      case, was found in cells of the B-lymphoid (CD19+) lineage but not in T cells 
      (CD3+) or in cells of the myeloid (CD13+) or erythroid (glycophorin A+) lineages, 
      indicative of a lymphoid committed progenitor cell. Heterogeneity of lineage 
      involvement was found in Ph+ ALL: the m-BCR Ph+ clone was found in lymphoid/blast 
      cells but not in neutrophils or eosinophils. In contrast both M-BCR Ph+ clones 
      were detected in myeloid as well as lymphoid lineages, indicative of a stem cell 
      origin.
FAU - Kasprzyk, A
AU  - Kasprzyk A
AD  - Cytogenetics Laboratory, The Royal Free and University College School of 
      Medicine, Pond Street, London NW3 2QG, UK.
FAU - Harrison, C J
AU  - Harrison CJ
FAU - Secker-Walker, L M
AU  - Secker-Walker LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
EIN - Leukemia 2000 Mar;14(3):5;26
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - *Diploidy
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - *Philadelphia Chromosome
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics/pathology
EDAT- 1999/12/22 09:00
MHDA- 2000/04/29 09:00
CRDT- 1999/12/22 09:00
PHST- 1999/12/22 09:00 [pubmed]
PHST- 2000/04/29 09:00 [medline]
PHST- 1999/12/22 09:00 [entrez]
AID - 10.1038/sj.leu.2401580 [doi]
PST - ppublish
SO  - Leukemia. 1999 Dec;13(12):2000-6. doi: 10.1038/sj.leu.2401580.