PMID- 10606164
OWN - NLM
STAT- MEDLINE
DCOM- 20000118
LR  - 20031114
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 17
IP  - 6
DP  - 1999
TI  - Age-dependent abnormalities of hematopoietic stem cells in (NZW x BXSB)F1 mice.
PG  - 357-65
AB  - The (NZW x BXSB)F1 (W/BF1) mouse is known as an autoimmune-prone strain which 
      develops lupus nephritis, thrombocytopenia due to platelet-specific 
      autoantibodies, leukocytosis, and myocardial infarction. In this experiment, we 
      investigated the age-dependent abnormalities of the hematopoietic stem cells 
      (HSCs) and hematopoiesis in this mouse. White blood cell counts (especially 
      Mac-1- or Gr-1-positive cells) in the peripheral blood of 12-week-old W/BF1 mice 
      increased in comparison with those of four-week-old W/BF1 or normal mice. To 
      investigate whether the abnormal hematopoiesis can be attributed to the HSCs of 
      W/BF1 mice, colony-forming unit in spleen (CFU-S) and colony-forming unit in 
      culture (CFU-C) assays were performed. Day 12 CFU-S counts of 12-week-old W/BF1 
      mice significantly increased in comparison with those of four-week-old W/BF1 mice 
      or normal mice. In the CFU-C assay, CFU-GEMM and CFU-GM counts in 12-week-old 
      W/BF1 mice increased in comparison with those of four-week-old W/BF1 or control 
      mice. The bone marrow cells (BMCs) from 12-week-old W/BF1 mice showed a high 
      level of G-CSF and a low level of GM-CSF in mRNA expression. To examine the 
      effect of HSCs from 12-week-old W/BF1 mice on the onset of autoimmune diseases 
      and the abnormal hematopoiesis, T- and B-cell-depleted BMCs of four-week-old or 
      12-week-old W/BF1 mice were transplanted to C3H mice. Recipient C3H mice that had 
      received the BMCs from 12-week-old W/BF1 mice showed an earlier onset of 
      autoimmune diseases and a shorter survival rate than those that had received the 
      BMCs from four-week-old W/BF1 mice. These data suggest that the HSCs from 
      12-week-old W/BF1 mice showing the symptoms of autoimmune diseases have the 
      capacity to induce autoimmune diseases earlier than the HSCs from four-week-old 
      W/BF1 mice.
FAU - Sugihara, A
AU  - Sugihara A
AD  - First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, 
      Japan.
FAU - Adachi, Y
AU  - Adachi Y
FAU - Inaba, M
AU  - Inaba M
FAU - Hisha, H
AU  - Hisha H
FAU - Sugiura, K
AU  - Sugiura K
FAU - Miyashima, S
AU  - Miyashima S
FAU - Amoh, Y
AU  - Amoh Y
FAU - Taketani, S
AU  - Taketani S
FAU - Oyaizu, H
AU  - Oyaizu H
FAU - Ikebukuro, K
AU  - Ikebukuro K
FAU - Kawamura, M
AU  - Kawamura M
FAU - Genba, H
AU  - Genba H
FAU - Horio, T
AU  - Horio T
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (RNA, Messenger)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Age of Onset
MH  - Aging/*pathology
MH  - Animals
MH  - Disease Models, Animal
MH  - Gene Expression/immunology
MH  - Granulocyte Colony-Stimulating Factor/genetics
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/genetics
MH  - Hematopoiesis/immunology
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/chemistry/*pathology
MH  - Leukocyte Count
MH  - Leukocytosis/mortality/pathology/therapy
MH  - Lupus Nephritis/mortality/*pathology/therapy
MH  - Macrophage Colony-Stimulating Factor/genetics
MH  - Macrophage-1 Antigen/analysis
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - Mice, Inbred NZB
MH  - Platelet Count
MH  - RNA, Messenger/analysis
MH  - Survival Analysis
MH  - Thrombocytopenia/mortality/*pathology/therapy
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1002/stem.170357 [doi]
PST - ppublish
SO  - Stem Cells. 1999;17(6):357-65. doi: 10.1002/stem.170357.