PMID- 10606178
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20091119
IS  - 0392-9078 (Print)
IS  - 0392-9078 (Linking)
VI  - 18
IP  - 3
DP  - 1999 Sep
TI  - N-acetylglucosaminyltransferase V activity in metastatic models of human 
      hepatocellular carcinoma in nude mice.
PG  - 331-5
AB  - N-linked beta 1-6 branched oligosaccharides may contribute directly to the 
      malignant phenotype including metastatic potential of tumour cells. Increased 
      beta 1-6 branching was associated with an increased level of 
      N-acetylglucosaminyltransferase V (GnT V). In this report, the tissues from two 
      metastatic models of human hepatocellular carcinoma (HCC) in nude mice were 
      obtained. GnT V activity and mRNA level were determined. Results showed that GnT 
      V activity in highly metastatic LCI-D20 models (Liver Cancer Institute, passage 
      time: 20 days) (413.1+/-86.4U) was much higher than that in low metastatic 
      LCI-D35 model (passage time 35 days) (155.3+/-31.9U). Northern blot showed that 
      the mRNA level of GnT V in two models had no change. During the selection of a 
      highly metastatic LCI-D20 model, GnT V activity increased from 301.6+/-57.3U to 
      413.1+/-86.4U while the highly metastatic LCI-D20 model acquired higher 
      metastatic ability after selection. When highly metastatic LCI-D20 model tissues 
      were implanted subcutaneously (s.c.), the GnT V activity decreased dramatically 
      from 413.1+/-86.4U to 94.9U. This is the first report that GnT V activity 
      increased in HCC during metastasis in vivo.
FAU - Shao, D M
AU  - Shao DM
AD  - Gene Research Center and Dept. of Biochemistry, Shanghai Medical University and 
      Key Laboratory of Glycoconjugate, Ministry of Public Health, P.R. China.
FAU - Wang, Q H
AU  - Wang QH
FAU - Chen, C
AU  - Chen C
FAU - Shen, Z H
AU  - Shen ZH
FAU - Yao, M
AU  - Yao M
FAU - Zhou, X D
AU  - Zhou XD
FAU - Tang, Z Y
AU  - Tang ZY
FAU - Gu, J X
AU  - Gu JX
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Clin Cancer Res
JT  - Journal of experimental & clinical cancer research : CR
JID - 8308647
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.4.1.- (N-Acetylglucosaminyltransferases)
RN  - EC 2.4.1.150 (N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Carbohydrate Sequence
MH  - Carcinoma, Hepatocellular/enzymology/*pathology
MH  - Enzyme Induction
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Liver Neoplasms/enzymology/*pathology
MH  - Lung Neoplasms/enzymology/secondary
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Molecular Sequence Data
MH  - N-Acetylglucosaminyltransferases/*analysis/biosynthesis/genetics
MH  - *Neoplasm Metastasis
MH  - Neoplasm Proteins/*analysis/biosynthesis/genetics
MH  - Neoplasm Transplantation
MH  - RNA, Messenger/biosynthesis
MH  - Selection, Genetic
MH  - Transplantation, Heterologous
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
PST - ppublish
SO  - J Exp Clin Cancer Res. 1999 Sep;18(3):331-5.