PMID- 10606893
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 107
IP  - 4
DP  - 1999 Dec
TI  - Phenotypic and functional characteristics of monocyte-derived dendritic cells 
      from patients with myelodysplastic syndromes.
PG  - 844-50
AB  - We have compared the phenotypic and functional characteristics of dendritic cells 
      (DC) generated in vitro from the peripheral blood mononuclear fraction of 
      myelodysplastic syndrome (MDS) patients (four refractory anaemia, four refractory 
      anaemia with excess of blasts) with DCs generated in a similar way from eight 
      healthy donors. After 10 d of culture in the presence of GM-CSF and IL-4, reduced 
      numbers and percentages of DCs were obtained in MDS subjects. MDS DCs exhibited 
      significantly lower expression of CD1a, CD54, CD80 and MHC class II molecules. 
      Their ability to stimulate T lymphocytes in an allogeneic mixed leucocyte 
      reaction was reduced in comparison to normal subjects. Furthermore, MDS DCs also 
      showed a reduced receptor-mediated endocytosis as demonstrated by FITC-dextran 
      uptake. Simultaneous fluorescence in situ hybridization (FISH) and 
      immunophenotypic analysis demonstrated that MDS DCs have the same cytogenetic 
      abnormality of the malignant clone. Taken together these findings indicate that, 
      in MDS, DCs are part of the malignant clone and exhibit a deficient antigen 
      uptake and presentation.
FAU - Matteo Rigolin, G
AU  - Matteo Rigolin G
AD  - Department of Haematological Medicine, Guy's, King's and St Thomas' School of 
      Medicine, London, U.K.
FAU - Howard, J
AU  - Howard J
FAU - Buggins, A
AU  - Buggins A
FAU - Sneddon, C
AU  - Sneddon C
FAU - Castoldi, G
AU  - Castoldi G
FAU - Hirst, W J
AU  - Hirst WJ
FAU - Mufti, G J
AU  - Mufti GJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Dendritic Cells/*pathology
MH  - Endocytosis/physiology
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocyte Culture Test, Mixed
MH  - Myelodysplastic Syndromes/*pathology
MH  - Phenotype
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - bjh1781 [pii]
AID - 10.1046/j.1365-2141.1999.01781.x [doi]
PST - ppublish
SO  - Br J Haematol. 1999 Dec;107(4):844-50. doi: 10.1046/j.1365-2141.1999.01781.x.