PMID- 10607310
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 50
IP  - 6
DP  - 1999 Dec
TI  - Expression and analysis of recombinant salmon parvalbumin, the major allergen in 
      Atlantic salmon (Salmo salar).
PG  - 619-25
AB  - The parvalbumin from white muscle of Atlantic salmon was previously found to be a 
      major allergen, and designated Sal s1. Two distinct cDNAs, 14.1 and 24.1, which 
      comprise the entire parvalbumin-encoding regions, were cloned, revealing 
      transcripts from two different parvalbumin genes. In the present study, the 
      protein-coding regions of these cDNAs were subcloned into an Escherichia coli 
      expression vector (pET-19b). Both proteins were expressed and the generated 
      target proteins were localized in both soluble and insoluble fractions of the 
      expression host. The recombinant products in the soluble fraction were purified 
      using the His tag-purification system and analysed on Western blots with 
      anti-salmon parvalbumin polyclonal rabbit sera and sera from patients allergic to 
      fish. Both recombinant products (His10-14.1 and His10-24.1) reacted positively 
      with salmon parvalbumin-specific immunoglobulin G (IgG) from rabbits, and with 
      specific immunoglobulin E (IgE) from the sera of six fish-allergic patients. The 
      allergenicity of His10-14.1 was confirmed using enzyme-linked immunosorbent assay 
      (ELISA). The 14.1 cDNA of salmon parvalbumin was shown to be the dominant type 
      represented in a muscle cDNA library.
FAU - Van Do, T
AU  - Van Do T
AD  - Allergy Research Group, Department of Clinical Biochemistry, Haukeland University 
      Hospital, Bergen, Norway.
FAU - Hordvik, I
AU  - Hordvik I
FAU - Endresen, C
AU  - Endresen C
FAU - Elsayed, S
AU  - Elsayed S
LA  - eng
SI  - GENBANK/X97825
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Allergens)
RN  - 0 (DNA, Complementary)
RN  - 0 (Dietary Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Muscle Proteins)
RN  - 0 (Parvalbumins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (allergen Sal s1)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/biosynthesis/genetics/*immunology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibody Affinity
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Dietary Proteins/biosynthesis/genetics/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Food Hypersensitivity/blood/*immunology
MH  - Gene Library
MH  - Humans
MH  - Immunoglobulin E/blood/immunology
MH  - Immunoglobulin G/immunology
MH  - Molecular Sequence Data
MH  - Muscle Proteins/biosynthesis/genetics/*immunology
MH  - Muscle, Skeletal/chemistry/immunology
MH  - Parvalbumins/biosynthesis/genetics/*immunology
MH  - Rabbits
MH  - Recombinant Fusion Proteins/biosynthesis/immunology/isolation & purification
MH  - Salmon/genetics/*immunology
MH  - Seafood/*adverse effects
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - sji637 [pii]
AID - 10.1046/j.1365-3083.1999.00637.x [doi]
PST - ppublish
SO  - Scand J Immunol. 1999 Dec;50(6):619-25. doi: 10.1046/j.1365-3083.1999.00637.x.