PMID- 10607828
OWN - NLM
STAT- MEDLINE
DCOM- 20000228
LR  - 20190513
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 9
IP  - 2
DP  - 2000 Jan 22
TI  - Human centromeres and neocentromeres show identical distribution patterns of >20 
      functionally important kinetochore-associated proteins.
PG  - 175-85
AB  - Using combined immunofluorescence and fluorescence in situ hybridization (FISH) 
      analysis we have extensively characterized the proteins associating with two 
      different homologue human neocentromeres at interphase and 
      prometaphase/metaphase, and compared these directly with those found with normal 
      human centromeres. Antisera to CENP-A, CENP-B, CENP-C, CENP-E, CENP-F, INCENP, 
      CLIP-170, dynein, dynactin subunits p150 (Glued) and Arp1, MCAK, Tsg24, p55CDC, 
      HZW10, HBUB1, HBUBR1, BUB3, MAD2, ERK1, 3F3/2, topoisomerase II and a murine HP1 
      homologue, M31, were used in immuno-fluorescence experiments in conjunction with 
      FISH employing specific DNA probes to clearly identify neocentromeric DNA. We 
      found that except for the total absence of CENP-B binding, neocentromeres are 
      indistinguishable from their alpha satellite-containing counterparts in terms of 
      protein composition and distribution. This suggests that the DNA base of a 
      potential centromeric locus is of minimal importance in determining the overall 
      structure of a functional kinetochore and that, once seeded, the events leading 
      to functional kinetochore formation occur independently of primary DNA sequence.
FAU - Saffery, R
AU  - Saffery R
AD  - The Murdoch Institute, Royal Children's Hospital, Flemington Road, Parkville 
      3052, Australia.
FAU - Irvine, D V
AU  - Irvine DV
FAU - Griffiths, B
AU  - Griffiths B
FAU - Kalitsis, P
AU  - Kalitsis P
FAU - Wordeman, L
AU  - Wordeman L
FAU - Choo, K H
AU  - Choo KH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Chromatin)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Immune Sera)
RN  - 0 (Microtubule-Associated Proteins)
SB  - IM
MH  - Anaphase/genetics
MH  - Animals
MH  - CHO Cells
MH  - Cell Cycle/genetics
MH  - Cell Line, Transformed
MH  - Centromere/immunology/*metabolism/physiology
MH  - Chromatin/metabolism
MH  - Chromosomal Proteins, Non-Histone/immunology/*metabolism/physiology
MH  - Chromosomes, Human, Pair 10/metabolism
MH  - Chromosomes, Human, Pair 20/metabolism
MH  - Cricetinae
MH  - Humans
MH  - Immune Sera/metabolism
MH  - Kinetochores/*metabolism/physiology
MH  - Metaphase/genetics
MH  - Microtubule-Associated Proteins/metabolism
MH  - Protein Binding/genetics
MH  - Tumor Cells, Cultured
EDAT- 1999/12/23 09:00
MHDA- 2000/03/04 09:00
CRDT- 1999/12/23 09:00
PHST- 1999/12/23 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 1999/12/23 09:00 [entrez]
AID - ddd025 [pii]
AID - 10.1093/hmg/9.2.175 [doi]
PST - ppublish
SO  - Hum Mol Genet. 2000 Jan 22;9(2):175-85. doi: 10.1093/hmg/9.2.175.