PMID- 10607865
OWN - NLM
STAT- MEDLINE
DCOM- 20000217
LR  - 20221207
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 385
IP  - 2-3
DP  - 1999 Dec 3
TI  - Involvement of the serotonin transporter in the formation of hydroxyl radicals 
      induced by 3,4-methylenedioxymethamphetamine.
PG  - 103-10
AB  - The mechanism of 3,4-methylenedioxymethamphetamine (MDMA)-induced depletion of 
      brain serotonin (5-hydroxytryptamine, 5-HT) has been proposed to involve the 
      generation of reactive oxygen species. In the present study, quantification of 
      the extracellular concentration of 2,3-dihydroxybenzoic acid (2,3-DHBA) from 
      salicylic acid was used as an index of hydroxyl radical generation. Although both 
      MDMA and D-amphetamine markedly increased the extracellular concentration of 
      dopamine in the striatum, only MDMA increased the extracellular concentration of 
      2,3-DHBA. Treatment with fluoxetine either 1 h prior to or 4 h following the 
      administration of MDMA reduced the MDMA-induced formation of 2,3-DHBA and also 
      attenuated the MDMA-induced depletion of 5-HT in the striatum. These results are 
      supportive of the view that the MDMA-induced generation of hydroxyl radicals and, 
      ultimately, the long-term depletion of 5-HT, is dependent, in part, on the 
      activation of the 5-HT transporter.
FAU - Shankaran, M
AU  - Shankaran M
AD  - College of Pharmacy, University of Cincinnati, 3223 Eden Avenue, Cincinnati, OH 
      45267-0004, USA.
FAU - Yamamoto, B K
AU  - Yamamoto BK
FAU - Gudelsky, G A
AU  - Gudelsky GA
LA  - eng
GR  - DA07427/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Carrier Proteins)
RN  - 0 (Dopamine Agents)
RN  - 0 (Hydroxybenzoates)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Serotonin Agents)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 0 (Serotonin Uptake Inhibitors)
RN  - 0 (Slc6a4 protein, rat)
RN  - 01K63SUP8D (Fluoxetine)
RN  - 333DO1RDJY (Serotonin)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 70D5FBB392 (2,3-dihydroxybenzoic acid)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - O414PZ4LPZ (Salicylic Acid)
RN  - TZ47U051FI (Dextroamphetamine)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*physiology
MH  - Corpus Striatum/drug effects/metabolism
MH  - Dextroamphetamine/pharmacology
MH  - Dopamine Agents/pharmacology
MH  - Fluoxetine/pharmacology
MH  - Hydroxybenzoates/metabolism
MH  - Hydroxyl Radical/*metabolism
MH  - Male
MH  - Membrane Glycoproteins/*physiology
MH  - *Membrane Transport Proteins
MH  - Microdialysis
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - *Nerve Tissue Proteins
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Salicylic Acid/metabolism
MH  - Serotonin/metabolism
MH  - Serotonin Agents/*pharmacology
MH  - Serotonin Plasma Membrane Transport Proteins
MH  - Selective Serotonin Reuptake Inhibitors/pharmacology
MH  - Time Factors
EDAT- 1999/12/23 09:00
MHDA- 2000/02/19 09:00
CRDT- 1999/12/23 09:00
PHST- 1999/12/23 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 1999/12/23 09:00 [entrez]
AID - S0014-2999(99)00728-1 [pii]
AID - 10.1016/s0014-2999(99)00728-1 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1999 Dec 3;385(2-3):103-10. doi: 10.1016/s0014-2999(99)00728-1.