PMID- 10611517
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190831
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 118
IP  - 1-2
DP  - 1999 Dec 10
TI  - Rescue of axotomized rubrospinal neurons by brain-derived neurotrophic factor 
      (BDNF) in the developing opossum, Didelphis virginiana.
PG  - 177-84
AB  - Many rubrospinal neurons die in developing opossums when their axon is cut at 
      thoracic levels of the spinal cord and in the present study we asked whether they 
      can be rescued by brain-derived neurotrophic factor (BDNF). Bilateral injections 
      of Fast Blue (FB) were made into the rostral lumbar cord to prelabel rubrospinal 
      neurons and 5 days later the rubrospinal tract was cut unilaterally by 
      hemisecting the thoracic cord. Immediately after hemisection, BDNF-soaked gelfoam 
      was placed into the lesion cavity. Since pilot data indicated that one 
      application of BDNF was not sufficient to produce a rescue effect, a second 
      application was made 7 days later. Seven days after the second application the 
      pups were killed by an overdose of anesthetic so that the red nucleus 
      contralateral and ipsilateral to the lesion site could be examined for labeled 
      neurons. The rubrospinal tract is almost entirely crossed, so the red nucleus 
      contralateral to the lesion contained many axotomized neurons, whereas the red 
      nucleus ipsilateral to it did not. Age-matched controls were subjected to the 
      same procedures, but the gelfoam applied to the lesion site in the experimental 
      animals was soaked only in the vehicle used to deliver BDNF. In all cases, 
      labeled neurons were fewer in number in the red nucleus contralateral to the 
      lesion than ipsilateral to it. It was of particular interest, however, that 
      labeled neurons contralateral to the lesion were more numerous in the animals 
      treated with BDNF than in the controls. We conclude that BDNF rescues at least 
      some rubrospinal neurons from axotomy-induced cell death in developing opossums 
      suggesting that loss of access to BDNF, and perhaps other neurotrophins, 
      contributes to failure of rubrospinal neurons to survive axotomy.
FAU - Wang, X M
AU  - Wang XM
AD  - Department of Anatomy and Medical Education, The Ohio State University, College 
      of Medicine, 333 West Tenth Avenue, Columbus, OH 43210, USA.
FAU - Terman, J R
AU  - Terman JR
FAU - Martin, G F
AU  - Martin GF
LA  - eng
GR  - NS10165/NS/NINDS NIH HHS/United States
GR  - NS25095/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Amidines)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (diamidino compound 253-50)
SB  - IM
MH  - Amidines
MH  - Animals
MH  - Animals, Newborn/growth & development/physiology
MH  - *Axotomy
MH  - Brain-Derived Neurotrophic Factor/administration & dosage/*pharmacology
MH  - Cell Survival/drug effects
MH  - Drug Administration Schedule
MH  - Fluorescent Dyes
MH  - Lumbosacral Region
MH  - Neurons/*drug effects/physiology
MH  - Opossums/growth & development/*physiology
MH  - Red Nucleus/cytology/*drug effects
MH  - Spinal Cord/cytology/*drug effects
EDAT- 1999/12/28 00:00
MHDA- 1999/12/28 00:01
CRDT- 1999/12/28 00:00
PHST- 1999/12/28 00:00 [pubmed]
PHST- 1999/12/28 00:01 [medline]
PHST- 1999/12/28 00:00 [entrez]
AID - S0165380699001480 [pii]
AID - 10.1016/s0165-3806(99)00148-0 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 1999 Dec 10;118(1-2):177-84. doi: 
      10.1016/s0165-3806(99)00148-0.