PMID- 10612427
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 156
IP  - 1-2
DP  - 1999 Oct 25
TI  - Responses to stable ectopic estrogen receptor-beta expression in a rat fibroblast 
      cell line.
PG  - 95-105
AB  - To examine activity of estrogen receptor-beta (ERbeta) independently of estrogen 
      receptor-alpha (ERalpha), retrovirus-mediated gene transfer was used to insert 
      rat ERbeta into a rat fibroblast cell line (rat-1) that does not ordinarily 
      express ER. Stable expression of ERbeta in rat-1 cells was validated and then 
      characterized by reverse-transcription polymerase chain-reaction (RT-PCR) 
      analysis to examine the effects of estradiol (E2) treatment on expression of 
      specific target mRNAs. Results were compared with rat-1 cells and a previously 
      constructed rat-1 + ERalpha cell line. Progesterone receptor mRNA was not 
      detected in rat-1 cells and was induced by E2 in both rat-1 + ERalpha and rat-1 + 
      ERbeta cells. Treatment with E2 resulted in an increased rate of cell 
      proliferation (P < 0.05) in rat-1 + ERalpha cells, but not in rat-1 or rat-1 + 
      ERbeta cells. Data confirm studies using transient ER expression demonstrating 
      that ERalpha and ERbeta have both discrete and overlapping activity within the 
      same cell type in the presence of the same ligand.
FAU - Cheng, J
AU  - Cheng J
AD  - Department of Infectious Diseases and Physiology, College of Veterinary Medicine, 
      Oklahoma State University, Stillwater 74078-2006, USA.
FAU - Malayer, J R
AU  - Malayer JR
LA  - eng
GR  - 34260/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Recombinant Proteins)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Cell Division
MH  - Cell Line
MH  - Estradiol/pharmacology
MH  - Estrogen Receptor alpha
MH  - Estrogen Receptor beta
MH  - Fibroblasts
MH  - Gene Expression Regulation/drug effects
MH  - Genes, Reporter
MH  - Promoter Regions, Genetic/drug effects
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Receptors, Estrogen/*genetics/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Restriction Mapping
MH  - Retroviridae
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transfection
EDAT- 1999/12/28 00:00
MHDA- 1999/12/28 00:01
CRDT- 1999/12/28 00:00
PHST- 1999/12/28 00:00 [pubmed]
PHST- 1999/12/28 00:01 [medline]
PHST- 1999/12/28 00:00 [entrez]
AID - S0303-7207(99)00134-3 [pii]
AID - 10.1016/s0303-7207(99)00134-3 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1999 Oct 25;156(1-2):95-105. doi: 
      10.1016/s0303-7207(99)00134-3.