PMID- 10612912
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20131121
IS  - 0971-5916 (Print)
IS  - 0971-5916 (Linking)
VI  - 110
DP  - 1999 Sep
TI  - Identification of the appropriate tissue from formalin fixed perinatal autopsy 
      material for chromosomal ploidy detection by interphase FISH.
PG  - 102-6
AB  - We have evaluated the suitability of different formalin fixed paraffin embedded 
      tissues i.e., brain, bone, liver and placenta, for fluorescence in situ 
      hybridization (FISH) efficiency and chromosomal ploidy detection employing 
      directly labelled repetitive sequence probes for chromosome X, Y and 1. The study 
      was carried out on four foetal autopsy specimens. Cells from 50 mu thick tissue 
      sections were dissociated before performing mono and multicolour FISH with 
      directly labelled probes. Hybridization efficiency was maximum with brain tissue 
      (81, 55 and 24% for mono, dual and triple colour FISH, respectively), followed by 
      bone (45 and 34% for mono and dual colour FISH), liver (38, 19 and 0% for mono, 
      dual and triple colour FISH) and placenta (14, 5 and 0% for mono, dual and triple 
      colour FISH). These results indicated that brain is the most efficient material 
      followed by bone, liver placenta for chromosome ploidy detection by FISH in 
      formalin fixed tissues.
FAU - Halder, A
AU  - Halder A
AD  - Department of Medical Genetics, Sanjay Gandhi Postgraduate Institute of Medical 
      Sciences, Lucknow.
FAU - Park, Y
AU  - Park Y
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Med Res
JT  - The Indian journal of medical research
JID - 0374701
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Autopsy
MH  - Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - *Ploidies
MH  - *Tissue Fixation
EDAT- 1999/12/29 00:00
MHDA- 1999/12/29 00:01
CRDT- 1999/12/29 00:00
PHST- 1999/12/29 00:00 [pubmed]
PHST- 1999/12/29 00:01 [medline]
PHST- 1999/12/29 00:00 [entrez]
PST - ppublish
SO  - Indian J Med Res. 1999 Sep;110:102-6.