PMID- 10613899
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 147
IP  - 7
DP  - 1999 Dec 27
TI  - Histone macroH2A1.2 relocates to the inactive X chromosome after initiation and 
      propagation of X-inactivation.
PG  - 1399-408
AB  - The histone macroH2A1.2 has been implicated in X chromosome inactivation on the 
      basis of its accumulation on the inactive X chromosome (Xi) of adult female 
      mammals. We have established the timing of macroH2A1.2 association with the Xi 
      relative to the onset of X-inactivation in differentiating murine embryonic stem 
      (ES) cells using immuno-RNA fluorescence in situ hybridization (FISH). Before 
      X-inactivation we observe a single macroH2A1.2-dense region in both 
      undifferentiated XX and XY ES cells that does not colocalize with X inactive 
      specific transcript (Xist) RNA, and thus appears not to associate with the X 
      chromosome(s). This pattern persists through early stages of differentiation, up 
      to day 7. Then the frequency of XY cells containing a macroH2A1.2-rich domain 
      declines. In contrast, in XX cells there is a striking relocalization of 
      macroH2A1.2 to the Xi. Relocalization occurs in a highly synchronized wave over a 
      2-d period, indicating a precisely regulated association. The timing of 
      macroH2A1.2 accumulation on the Xi suggests it is not necessary for the 
      initiation or propagation of random X-inactivation.
FAU - Mermoud, J E
AU  - Mermoud JE
AD  - X-Inactivation Group, Medical Research Council Clinical Sciences Centre, Imperial 
      College School of Medicine, Hammersmith Hospital, London W12 0NN, United Kingdom. 
      jmermoud@hgmp.mrc.ac.uk
FAU - Costanzi, C
AU  - Costanzi C
FAU - Pehrson, J R
AU  - Pehrson JR
FAU - Brockdorff, N
AU  - Brockdorff N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Histones)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
RN  - 0 (Transcription Factors)
RN  - 0 (XIST non-coding RNA)
RN  - 0 (macroH2A histone)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/genetics
MH  - Cell Line
MH  - *Dosage Compensation, Genetic
MH  - Female
MH  - Histones/*metabolism
MH  - Male
MH  - Mice
MH  - RNA/metabolism
MH  - RNA, Long Noncoding
MH  - *RNA, Untranslated
MH  - Sex Chromatin/metabolism
MH  - Stem Cells/cytology/metabolism
MH  - Transcription Factors/genetics/metabolism
MH  - Tumor Cells, Cultured
MH  - X Chromosome/genetics/*metabolism
MH  - Y Chromosome/genetics
PMC - PMC2174253
EDAT- 1999/12/30 00:00
MHDA- 1999/12/30 00:01
PMCR- 2000/06/27
CRDT- 1999/12/30 00:00
PHST- 1999/12/30 00:00 [pubmed]
PHST- 1999/12/30 00:01 [medline]
PHST- 1999/12/30 00:00 [entrez]
PHST- 2000/06/27 00:00 [pmc-release]
AID - 9907065 [pii]
AID - 10.1083/jcb.147.7.1399 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Dec 27;147(7):1399-408. doi: 10.1083/jcb.147.7.1399.